pSEL1

23011 Mus musculus Bacterial Expression

Plasmid Information

Depositor
James Bowie
Article
Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.
Insert
DHFR (Dhfr Mouse)
Tags
6 His Tag (not in frame) and Xpress epitope (not …
Available Since
March 10, 2010
Availability
Available

Sequence Information

Length
714 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 312..336 Rev
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 339..372 Rev
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
6xHis 6xHis affinity tag 375..393 Rev
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
araBAD promoter araBAD 438..604 Rev
DNA Sequence
AAAGCCATGACAAAAACGCGTAACAAAAGTGTCTATAATCACGGCAGAAAAGTCCACATTGATTATTTGCACGGCGTCACACTTTGCTATGCCATAGCATTTTTATCCATAAGATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCAT