pKESaPar

211829 Synthetic Bacterial Expression

Plasmid Information

Purpose
Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.
Depositor
Anita Emmerstorfer-Augustin
Article
Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do
Insert
eGFP
Promoter
T5
Available Since
Feb. 13, 2024
Availability
Available

Sequence Information

Length
6,890 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 4
Label Gene Product Position Strand
EGFP the original enhanced GFP (Yang et al.,… 124..844 Fwd
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
EGFP the original enhanced GFP (Yang et al.,… 124..841 Fwd
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
KanR aph(3')-Ia aminoglycoside phosphotransferase 1353..2169 Fwd
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
RepA repA replication protein of plasmid pSa 2838..3810 Rev
DNA Sequence
ATGCCTAAGAACAACAAAGCCCCCGGCCATCGTATCAACGAGATCATCAAGACGAGCCTCGCGCTCGAAATGGAGGATGCCCGCGAAGCTGGCTTAGTCGGCTACATGGCCCGTTGCCTTGTGCAAGCGACCATGCCCCACACCGACCCCAAGACCAGCAACTTTGAGCGCACCAATGGCATCGTCACCTTGTCGATCATGGGCAAGCCGAGCATCGGCCTGCCCTACGGTTCTATGCCGCGCACCTTGCTTGCTTGGATATGCACCGAGGCCGTGCGAACGAAAGACCCCGTGTTGAACCTTGGCCGGTCGCAATCGGAATTTCTACAAAGGCTCGGAATGCACACCGATGGCCGTTACACGGCCACCCTTCGCAATCAGGCGCAACGCCTGTTTTCATCCATGATTTCGCTTGCCGGCGAGCAAGGCAATGACTTCGGCATTGAGAACGTCGTCATTGCCAAGCGCGCTTTTCTATTCTGGAATCCCAAGCGGCCAGAAGATCGGGCGCTATGGGATAGCACCCTCACCCTCACAGGCGATTTCTTCGAGGAAGTCACCCGCTCACCGGTTCCTATCCGAATCGACTACCTGCATGCCTTGCGGCAGTCTCCGCTTGCGATGGACATTTACACGTGGCTGACCTATCGCGTGTTCCTGTTGCGGGCCAAGGGCCGCCCCTTCGTGCAAATCCCTTGGGTCGCCCTGCAAGCGCAATTCGGCTCATCCTATGGCAGCCGCGCACGCAACTCGCCCGAACTGGACGATAAGGCCCGAGAGCGGGCAGAGCGGGCAGCACTCGCCAGCTTCAAATACAACTTCAAAAAGCGCCTACGCGAAGTGTTGATTGTCTATCCCGAGGCAAGCGACTGCATCGAAGATGACGGCGAATGCCTGCGCATCAAATCCACACGCCTGCATGTCACCCGCGCACCCGGCAAGGGCGCTCGCATCGGCCCCCCTCCGACTTGA
Protein Sequence
MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATMPHTDPKTSNFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRSQSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNPKRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLRAKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREVLIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT
promoter 1
Label Gene Product Position Strand
T5 promoter 10..55 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 1
Label Gene Product Position Strand
lac operator 30..47 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
pSa ori 2366..2802 Fwd
DNA Sequence
GCGCTCGACGGTGGGCAACCGCTTGCGGTTGTCCACGGGCGGAGCCGGTGCGCGTAGCGCATTGTCCACAAGCCAAGGGCGACCAATAATTGATATATATATTCATAATTGAAAAGCTAATTGAACATACTACTTGCTGTAACTACTTGCCGGAGCGAGGGGTGTTTGCAAGCTGTTGATCTGAAAGGGCTATTAGCGTTCTCACGTGCCTTTTTGATTAGCGATTTCACGTGACCTTATTAGCGATTTCACGTACTCCGATTAGCGATTTCACGTACCCTGATTAGCGATTTCACGTGGATAGTTTTTGGAGCGGGCCGGAAAGCCCCGTGAATCAAGGCTTTGCGGGGCATTAGCGGTTTCACGTGGATAACTACCCTCTATCCACAGGCTTCCGGGGATAAAAAAGCCCGCTCGACGGCGGGCTGTTGGATGG
terminator 1
Label Gene Product Position Strand
rrnB T2 terminator 1217..1245 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT