pKESa

211828 Synthetic Bacterial Expression

Plasmid Information

Purpose
Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.
Depositor
Anita Emmerstorfer-Augustin
Article
Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do
Insert
eGFP
Promoter
T5
Available Since
Feb. 13, 2024
Availability
Available

Sequence Information

Length
4,648 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
EGFP — the original enhanced GFP (Yang et al.,… 0..4648 Fwd
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
KanR aph(3')-Ia aminoglycoside phosphotransferase 823..1639 Fwd
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
RepA repA replication protein of plasmid pSa 2308..3280 Rev
DNA Sequence
ATGCCTAAGAACAACAAAGCCCCCGGCCATCGTATCAACGAGATCATCAAGACGAGCCTCGCGCTCGAAATGGAGGATGCCCGCGAAGCTGGCTTAGTCGGCTACATGGCCCGTTGCCTTGTGCAAGCGACCATGCCCCACACCGACCCCAAGACCAGCAACTTTGAGCGCACCAATGGCATCGTCACCTTGTCGATCATGGGCAAGCCGAGCATCGGCCTGCCCTACGGTTCTATGCCGCGCACCTTGCTTGCTTGGATATGCACCGAGGCCGTGCGAACGAAAGACCCCGTGTTGAACCTTGGCCGGTCGCAATCGGAATTTCTACAAAGGCTCGGAATGCACACCGATGGCCGTTACACGGCCACCCTTCGCAATCAGGCGCAACGCCTGTTTTCATCCATGATTTCGCTTGCCGGCGAGCAAGGCAATGACTTCGGCATTGAGAACGTCGTCATTGCCAAGCGCGCTTTTCTATTCTGGAATCCCAAGCGGCCAGAAGATCGGGCGCTATGGGATAGCACCCTCACCCTCACAGGCGATTTCTTCGAGGAAGTCACCCGCTCACCGGTTCCTATCCGAATCGACTACCTGCATGCCTTGCGGCAGTCTCCGCTTGCGATGGACATTTACACGTGGCTGACCTATCGCGTGTTCCTGTTGCGGGCCAAGGGCCGCCCCTTCGTGCAAATCCCTTGGGTCGCCCTGCAAGCGCAATTCGGCTCATCCTATGGCAGCCGCGCACGCAACTCGCCCGAACTGGACGATAAGGCCCGAGAGCGGGCAGAGCGGGCAGCACTCGCCAGCTTCAAATACAACTTCAAAAAGCGCCTACGCGAAGTGTTGATTGTCTATCCCGAGGCAAGCGACTGCATCGAAGATGACGGCGAATGCCTGCGCATCAAATCCACACGCCTGCATGTCACCCGCGCACCCGGCAAGGGCGCTCGCATCGGCCCCCCTCCGACTTGA
Protein Sequence
MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATMPHTDPKTSNFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRSQSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNPKRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLRAKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREVLIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT
promoter 1
Label Gene Product Position Strand
T5 promoter — — 4128..4173 Fwd
DNA Sequence
TCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATA
protein_bind 1
Label Gene Product Position Strand
lac operator — — 4148..4165 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
pSa ori — — 1836..2272 Fwd
DNA Sequence
GCGCTCGACGGTGGGCAACCGCTTGCGGTTGTCCACGGGCGGAGCCGGTGCGCGTAGCGCATTGTCCACAAGCCAAGGGCGACCAATAATTGATATATATATTCATAATTGAAAAGCTAATTGAACATACTACTTGCTGTAACTACTTGCCGGAGCGAGGGGTGTTTGCAAGCTGTTGATCTGAAAGGGCTATTAGCGTTCTCACGTGCCTTTTTGATTAGCGATTTCACGTGACCTTATTAGCGATTTCACGTACTCCGATTAGCGATTTCACGTACCCTGATTAGCGATTTCACGTGGATAGTTTTTGGAGCGGGCCGGAAAGCCCCGTGAATCAAGGCTTTGCGGGGCATTAGCGGTTTCACGTGGATAACTACCCTCTATCCACAGGCTTCCGGGGATAAAAAAGCCCGCTCGACGGCGGGCTGTTGGATGG
terminator 1
Label Gene Product Position Strand
rrnB T2 terminator — — 687..715 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT