pESC-URA-GFP-Ubc9ts

20369 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Depositor
Judith Frydman
Article
Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.
Insert
SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
Tags
GFP
Mutation
Y68L This mutation renders Ubc9 temperature-sens…
Available Since
May 28, 2009
Availability
Available

Sequence Information

Length
793 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 47..65 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
Myc Myc (human c-Myc proto-oncogene) epitop… 545..575 Fwd
DNA Sequence
GAACAAAAACTTATTTCTGAAGAAGATCTG
Protein Sequence
EQKLISEEDL
S-Tag affinity and epitope tag derived from p… 599..644 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS