| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 445..463 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
|
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| T7 tag (gene 10 leader) | — | leader peptide from bacteriophage T7 ge… | 466..499 | Fwd | |
|
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
|
|||||
| Xpress(TM) tag | — | Xpress(TM) epitope tag, including an en… | 502..526 | Fwd | |
|
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
|
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| LuxB | luxB | LuxB luciferase subunit | 532..1513 | Fwd | |
|
DNA Sequence
ATGAAATTTGGATTGTTCTTCCTTAACTTCATCAATTCAACAACTGTTCAAGAACAAAGTATAGTTCGCATGCAGGAAATAACGGAGTATGTTGATAAGTTGAATTTTGAACAGATTTTAGTGTATGAAAATCATTTTTCAGATAATGGTGTTGTCGGCGCTCCTCTGACTGTTTCTGGTTTTCTGCTCGGTTTAACAGAGAAAATTAAAATTGGTTCATTAAATCACATCATTACAACTCATCATCCTGTCCGCATAGCGGAGGAAGCTTGCTTATTGGATCAGTTAAGTGAAGGGAGATTTATTTTAGGGTTTAGTGATTGCGAAAAAAAAGATGAAATGCATTTTTTTAATCGCCCGGTTGAATATCAACAGCAACTATTTGAAGAGTGTTATGAAATCATTAACGATGCTTTAACAACAGGCTATTGTAATCCAGATAACGATTTTTATAGCTTCCCTAAAATATCTGTAAATCCCCATGCTTATACGCCAGGCGGACCTCGGAAATATGTAACAGCAACCAGTCATCATATTGTTGAGTGGGCGGCCAAAAAAGGTATTCCTCTCATCTTTAAGTGGGATGATTCTAATGATGTTAGATATGAATATGCTGAAAGATATAAAGCCGTTGCGGATAAATATGACGTTGACCTATCAGAGATAGACCATCAGTTAATGATATTAGTTAACTATAACGAAGATAGTAATAAAGCTAAACAAGAGACGCGTGCATTTATTAGTGATTATGTTCTTGAAATGCACCCTAATGAAAATTTCGAAAATAAACTTGAAGAAATAATTGCAGAAAACGCTGTCGGAAATTATACGGAGTGTATAACTGCGGCTAAGTTGGCAATTGAAAAGTGTGGTGCGAAAAGTGTATTGCTGTCCTTTGAACCAATGAATGATTTGATGAGCCAAAAAAATGTAATCAATATTGTTGATGATAATATTAAGAAGTACCACATGGAATATACC
Protein Sequence
MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHFSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGFSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYTPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEIDHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITAAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT
|
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| Venus | — | yellow fluorescent protein (YFP) with f… | 1519..2239 | Fwd | |
|
DNA Sequence
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGCTGATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
|
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| LuxA | luxA | LuxA luciferase subunit | 2390..3473 | Fwd | |
|
DNA Sequence
ATGAAATTTGGAAACTTTTTGCTTACATACCAACCTCCCCAATTTTCTCAAACAGAGGTAATGAAACGTTTGGTTAAATTAGGTCGCATCTCTGAGGAGTGTGGTTTTGATACCGTATGGTTACTGGAGCATCATTTCACGGAGTTTGGTTTGCTTGGTAACCCTTATGTCGCTGCTGCATATTTACTTGGCGCGACTAAAAAATTGAATGTAGGAACTGCCGCTATTGTTCTTCCCACAGCCCATCCAGTACGCCAACTTGAAGATGTGAATTTATTGGATCAAATGTCAAAAGGACGATTTCGGTTTGGTATTTGCCGAGGGCTTTACAACAAGGACTTTCGCGTATTCGGCACAGATATGAATAACAGTCGCGCCTTAGCGGAATGCTGGTACGGGCTGATAAAGAATGGCATGACAGAGGGATATATGGAAGCTGATAATGAACATATCAAGTTCCATAAGGTAAAAGTAAACCCCGCGGCGTATAGCAGAGGTGGCGCACCGGTTTATGTGGTGGCTGAATCAGCTTCGACGACTGAGTGGGCTGCTCAATTTGGCCTACCGATGATATTAAGTTGGATTATAAATACTAACGAAAAGAAAGCACAACTTGAGCTTTATAATGAAGTGGCTCAAGAATATGGGCACGATATTCATAATATCGACCATTGCTTATCATATATAACATCTGTAGATCATGACTCAATTAAAGCGAAAGAGATTTGCCGGAAATTTCTGGGGCATTGGTATGATTCTTATGTGAATGCTACGACTATTTTTGATGATTCAGACCAAACAAGAGGTTATGATTTCAATAAAGGGCAGTGGCGTGACTTTGTATTAAAAGGACATAAAGATACTAATCGCCGTATTGATTACAGTTACGAAATCAATCCCGTGGGAACGCCGCAGGAATGTATTGACATAATTCAAAAAGACATTGATGCTACAGGAATATCAAATATTTGTTGTGGATTTGAAGCTAATGGAACAGTAGACGAAATTATTGCTTCCATGAAGCTCTTCCAGTCTGATGTCATGCCATTTCTTAAAGAAAAACAACGTTCGCTATTATATTAG
Protein Sequence
MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHHFTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFGICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAYSRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNIDHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFVLKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASMKLFQSDVMPFLKEKQRSLLY
|
|||||
| AmpR | bla | beta-lactamase | 4288..5149 | Fwd | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
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| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 353..372 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| T7 promoter | — | — | 2304..2323 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| AmpR promoter | bla | — | 4183..4288 | Fwd | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 2323..2348 | Fwd | |
|
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 0..5810 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| f1 ori | — | — | 3702..4158 | Fwd | |
|
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 3558..3606 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
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