pCMU-4

172353 Synthetic Bacterial Expression

Plasmid Information

Purpose
CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA
Depositor
Eriko Takano
Article
Ye et al J Ind Microbiol Biotechnol. 2020 May;47(
Insert
D4-TheoR-cas9
Promoter
D4
Available Since
Dec. 10, 2021
Availability
Available

Sequence Information

Length
10,874 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 6
Label Gene Product Position Strand
pSG5 Rep rep replication initiator protein from the … 406..1849 Rev
DNA Sequence
ATGTGGAAGCCGGGCGAGGCGACGTGGGGCAACACGTGCCGCTGCAACAACGTCCACACCTGCCCGTGGTGCATGAGCCGCATCCTGGCCGTACGGGGCTCGAACGTGCAGCTCGCCGCCGACGGTCTCGCGGACGCCGGGTACGGGCTGCACCTTGGCACGAACACGTTGCGCCACTTCGAGCGCATGGCGTTCGGCACCGTCCGCAAGGGAATGCGGCACGGCCTGGTCGCGGTCTTGCACGACGGCTGGAAGGGCGCGTACGGCTCGTCCGGCCGCCGCTGGCGCACGATGCGCGACGACTTCGGGATCATCGGCTACGAGCGGGCCTTCGAGGACACCTTCGGCTGGGGCTCCGGCTGGCACCTGCACTGGCACACGCTCTGGGTCACCCGCGAGGTGCTCGGCCCGGACGCCCAGGCCGCGTTCCGCGACGCACTCGCCGGAGCCTGGGCCGCCGGGGTCGAATCCGCGGGCGGCTACACCGTCAGCGAGACGTGCGACCGGCCCGGTTGCTCCTGTGAGGGCAAGGGCCACGGCACCGACGTGCGCCCACTCAACGGCGCGGACGCCGCTGACGGCGATGCGGGCAAGCAGGCCCGCTACCTCTACAAGGACGGCGACAAGACCAAGGGCGGCGTCGCCAAGATCGGCCTGGAGCTGGCCGGACAGAACTTCAAGGCCGGTCGCGGCGACGACCGCATGGGACCGCTCGACCTGGGCGACGCGGCGGCCGCCGAGCTGCAGCGGCTTCGTCGGCCCGGCCCGTTCGTCGAGAAGTACCGCGAGCGCGAGTTCGGCGTGTTCCAGGTCCGCAAGCACTACCGGTCGCAGAACCTGAACCGGCTGATCAAGGAACTCGGCATCCAGCAGGACGTCCGCACCGAGGAGGAGATCACCGACGACACCGAGGGCCTGGTCGCCATCGCGGTCATCCCGGCCTACATCTGGTACCGATACATCGCCCGCGTCGCCGGACGTCGCCTCGACCTCATCAAGGTCGCCGAGACGTACGGCCTACCGGGCGTCCGGCGGCTCGTCGAGTCCTGGGGCCTGGTGTGGGGCAAGGACGTCCTGGACCCGCCCGCCCCGGAAGCGCCGGCCGCGCCGGGCGACCTTGACGCTGACCAGATGAGGTTCGAGGTCATGTCCGAGGAGGAAGCTGCTTTCCGCGAGGCGCGCCGGAAGGCCAACGAGGCCCGCACGGAGGAGCTGGCCGCTTCGCTCGACCGAGTGCGTCAGCCGAAGAAGGAGGCGATCCGCCCGACGATCAGCCTTCGGAAGCGCCTGAAGCCGAAGCCGGTCACCGTCGACGTCAAGACTCCCCCGCCCGGCGCCGCGTCGCCCGTGTGCCGCCGCTGCAAGGGCAAGCTCGCCCCGGTACTCCAGCCCTGGGGCCAGCACCCCGGAGACTGCCTCCGCGTCGACACAGCGGTCGCGTAG
Protein Sequence
MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLADAGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGIIGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTVSETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELAGQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLNRLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGLPGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANEARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKLAPVLQPWGQHPGDCLRVDTAVA
traJ traJ oriT-recognizing protein 3030..3402 Fwd
DNA Sequence
ATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCAAGGTGTACTGCCTTCCAGACGAACGAAGAGCGATTGAGGAAAAGGCGGCGGCGGCCGGCATGAGCCTGTCGGCCTACCTGCTGGCCGTCGGCCAGGGCTACAAAATCACGGGCGTCGTGGACTATGAGCACGTCCGCGAGCTGGCCCGCATCAATGGCGACCTGGGCCGCCTGGGCGGCCTGCTGAAACTCTGGCTCACCGACGACCCGCGCACGGCGCGGTTCGGTGATGCCACGATCCTCGCCCTGCTGGCGAAGATCGAAGAGAAGCAGGACGAGCTTGGCAAGGTCATGATGGGCGTGGTCCGCCCGAGGGCAGAGCCATGA
Protein Sequence
MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 3528..7635 Fwd
DNA Sequence
ATGGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACAGCGTGGGCTGGGCGGTCATCACCGACGAGTACAAGGTCCCCTCCAAGAAGTTCAAGGTCCTGGGCAACACCGACCGGCACTCGATCAAGAAGAACCTGATCGGCGCCCTGCTCTTCGACAGCGGCGAAACCGCCGAGGCGACCCGCCTGAAGCGGACCGCCCGTCGCCGCTACACCCGGCGCAAGAACCGCATCTGCTACCTGCAGGAGATCTTCTCCAACGAGATGGCCAAGGTCGACGACTCGTTCTTCCACCGGCTCGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGCCACCCGATCTTCGGCAACATCGTCGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTCCGCAAGAAGCTGGTCGACTCGACCGACAAGGCGGACCTGCGGCTCATCTACCTGGCCCTCGCGCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCCGACGTCGACAAGCTCTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTCGACGCCAAGGCGATCCTCTCCGCGCGCCTGAGCAAGTCCCGGCGCCTGGAGAACCTCATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTGTCGCTCGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCGAAGCTCCAGCTGTCCAAGGACACCTACGACGACGACCTGGACAACCTGCTCGCCCAGATCGGCGACCAGTACGCGGACCTCTTCCTGGCCGCGAAGAACCTCTCGGACGCCATCCTGCTCAGCGACATCCTGCGGGTCAACACCGAGATCACCAAGGCCCCGCTGTCGGCGAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTCAAGGCCCTCGTGCGCCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCGTCGCAGGAGGAGTTCTACAAGTTCATCAAGCCGATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTCGTCAAGCTGAACCGCGAGGACCTGCTCCGCAAGCAGCGGACCTTCGACAACGGCTCCATCCCGCACCAGATCCACCTGGGCGAGCTCCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGCGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCGTACTACGTCGGCCCCCTGGCCCGCGGCAACTCCCGGTTCGCGTGGATGACCCGGAAGTCGGAGGAAACCATCACCCCGTGGAACTTCGAGGAGGTCGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGCATGACCAACTTCGACAAGAACCTCCCGAACGAGAAGGTCCTGCCCAAGCACAGCCTGCTCTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTCAAGTACGTGACCGAGGGCATGCGGAAGCCGGCCTTCCTGTCCGGCGAGCAGAAGAAGGCGATCGTCGACCTGCTCTTCAAGACCAACCGCAAGGTCACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCCGTCGAGATCTCGGGCGTGGAGGACCGCTTCAACGCCTCCCTGGGCACCTACCACGACCTGCTCAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTCCTCACCCTGACCCTCTTCGAGGACCGCGAGATGATCGAGGAGCGGCTCAAGACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGCCGGTACACCGGCTGGGGCCGCCTCTCCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTCAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTCATCCACGACGACTCGCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGTCCGGCCAGGGCGACAGCCTCCACGAGCACATCGCCAACCTGGCGGGCTCCCCGGCGATCAAGAAGGGCATCCTCCAGACCGTCAAGGTCGTGGACGAGCTGGTCAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACTCCCGCGAGCGGATGAAGCGCATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTGAAGGAGCACCCGGTCGAGAACACCCAGCTCCAGAACGAGAAGCTGTACCTCTACTACCTGCAGAACGGCCGCGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGAGCGACTACGACGTCGACCACATCGTGCCGCAGTCCTTCCTGAAGGACGACTCGATCGACAACAAGGTCCTGACCCGCTCCGACAAGAACCGGGGCAAGTCCGACAACGTGCCCTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGCCAGCTGCTCAACGCCAAGCTCATCACCCAGCGCAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGTCGGAGCTCGACAAGGCGGGCTTCATCAAGCGCCAGCTCGTCGAAACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTGATCACCCTCAAGAGCAAGCTGGTGTCCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTCCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCCGTCGTGGGCACCGCGCTGATCAAGAAGTACCCGAAGCTGGAGTCCGAGTTCGTCTACGGCGACTACAAGGTCTACGACGTGCGCAAGATGATCGCCAAGTCGGAGCAGGAGATCGGCAAGGCCACCGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGCAAGCGGCCCCTGATCGAAACCAACGGCGAAACCGGCGAGATCGTCTGGGACAAGGGCCGCGACTTCGCCACCGTCCGGAAGGTGCTGTCCATGCCGCAGGTCAACATCGTCAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTCCCCAAGCGCAACTCGGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCGAAGAAGTACGGCGGCTTCGACAGCCCCACCGTCGCCTACTCCGTGCTGGTCGTGGCGAAGGTCGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTCGGCATCACCATCATGGAGCGCTCCTCGTTCGAGAAGAACCCGATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTCAAGAAGGACCTCATCATCAAGCTGCCCAAGTACTCGCTGTTCGAGCTCGAGAACGGCCGCAAGCGGATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTCCCGTCCAAGTACGTCAACTTCCTGTACCTCGCGTCCCACTACGAGAAGCTGAAGGGCTCGCCCGAGGACAACGAGCAGAAGCAGCTCTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCTCGGAGTTCAGCAAGCGGGTCATCCTGGCCGACGCGAACCTCGACAAGGTGCTGTCCGCCTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCGGAGAACATCATCCACCTGTTCACCCTCACCAACCTGGGCGCCCCCGCCGCGTTCAAGTACTTCGACACCACCATCGACCGCAAGCGGTACACCAGCACCAAGGAGGTCCTCGACGCGACCCTGATCCACCAGTCCATCACCGGCCTGTACGAAACCCGCATCGACCTCTCCCAGCTCGGCGGCGACTGA
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 3528..7632 Fwd
DNA Sequence
ATGGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACAGCGTGGGCTGGGCGGTCATCACCGACGAGTACAAGGTCCCCTCCAAGAAGTTCAAGGTCCTGGGCAACACCGACCGGCACTCGATCAAGAAGAACCTGATCGGCGCCCTGCTCTTCGACAGCGGCGAAACCGCCGAGGCGACCCGCCTGAAGCGGACCGCCCGTCGCCGCTACACCCGGCGCAAGAACCGCATCTGCTACCTGCAGGAGATCTTCTCCAACGAGATGGCCAAGGTCGACGACTCGTTCTTCCACCGGCTCGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGCCACCCGATCTTCGGCAACATCGTCGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTCCGCAAGAAGCTGGTCGACTCGACCGACAAGGCGGACCTGCGGCTCATCTACCTGGCCCTCGCGCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCCGACGTCGACAAGCTCTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTCGACGCCAAGGCGATCCTCTCCGCGCGCCTGAGCAAGTCCCGGCGCCTGGAGAACCTCATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTGTCGCTCGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCGAAGCTCCAGCTGTCCAAGGACACCTACGACGACGACCTGGACAACCTGCTCGCCCAGATCGGCGACCAGTACGCGGACCTCTTCCTGGCCGCGAAGAACCTCTCGGACGCCATCCTGCTCAGCGACATCCTGCGGGTCAACACCGAGATCACCAAGGCCCCGCTGTCGGCGAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTCAAGGCCCTCGTGCGCCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCGTCGCAGGAGGAGTTCTACAAGTTCATCAAGCCGATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTCGTCAAGCTGAACCGCGAGGACCTGCTCCGCAAGCAGCGGACCTTCGACAACGGCTCCATCCCGCACCAGATCCACCTGGGCGAGCTCCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGCGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCGTACTACGTCGGCCCCCTGGCCCGCGGCAACTCCCGGTTCGCGTGGATGACCCGGAAGTCGGAGGAAACCATCACCCCGTGGAACTTCGAGGAGGTCGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGCATGACCAACTTCGACAAGAACCTCCCGAACGAGAAGGTCCTGCCCAAGCACAGCCTGCTCTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTCAAGTACGTGACCGAGGGCATGCGGAAGCCGGCCTTCCTGTCCGGCGAGCAGAAGAAGGCGATCGTCGACCTGCTCTTCAAGACCAACCGCAAGGTCACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCCGTCGAGATCTCGGGCGTGGAGGACCGCTTCAACGCCTCCCTGGGCACCTACCACGACCTGCTCAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTCCTCACCCTGACCCTCTTCGAGGACCGCGAGATGATCGAGGAGCGGCTCAAGACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGCCGGTACACCGGCTGGGGCCGCCTCTCCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTCAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTCATCCACGACGACTCGCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGTCCGGCCAGGGCGACAGCCTCCACGAGCACATCGCCAACCTGGCGGGCTCCCCGGCGATCAAGAAGGGCATCCTCCAGACCGTCAAGGTCGTGGACGAGCTGGTCAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACTCCCGCGAGCGGATGAAGCGCATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTGAAGGAGCACCCGGTCGAGAACACCCAGCTCCAGAACGAGAAGCTGTACCTCTACTACCTGCAGAACGGCCGCGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGAGCGACTACGACGTCGACCACATCGTGCCGCAGTCCTTCCTGAAGGACGACTCGATCGACAACAAGGTCCTGACCCGCTCCGACAAGAACCGGGGCAAGTCCGACAACGTGCCCTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGCCAGCTGCTCAACGCCAAGCTCATCACCCAGCGCAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGTCGGAGCTCGACAAGGCGGGCTTCATCAAGCGCCAGCTCGTCGAAACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTGATCACCCTCAAGAGCAAGCTGGTGTCCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTCCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCCGTCGTGGGCACCGCGCTGATCAAGAAGTACCCGAAGCTGGAGTCCGAGTTCGTCTACGGCGACTACAAGGTCTACGACGTGCGCAAGATGATCGCCAAGTCGGAGCAGGAGATCGGCAAGGCCACCGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGCAAGCGGCCCCTGATCGAAACCAACGGCGAAACCGGCGAGATCGTCTGGGACAAGGGCCGCGACTTCGCCACCGTCCGGAAGGTGCTGTCCATGCCGCAGGTCAACATCGTCAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTCCCCAAGCGCAACTCGGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCGAAGAAGTACGGCGGCTTCGACAGCCCCACCGTCGCCTACTCCGTGCTGGTCGTGGCGAAGGTCGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTCGGCATCACCATCATGGAGCGCTCCTCGTTCGAGAAGAACCCGATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTCAAGAAGGACCTCATCATCAAGCTGCCCAAGTACTCGCTGTTCGAGCTCGAGAACGGCCGCAAGCGGATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTCCCGTCCAAGTACGTCAACTTCCTGTACCTCGCGTCCCACTACGAGAAGCTGAAGGGCTCGCCCGAGGACAACGAGCAGAAGCAGCTCTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCTCGGAGTTCAGCAAGCGGGTCATCCTGGCCGACGCGAACCTCGACAAGGTGCTGTCCGCCTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCGGAGAACATCATCCACCTGTTCACCCTCACCAACCTGGGCGCCCCCGCCGCGTTCAAGTACTTCGACACCACCATCGACCGCAAGCGGTACACCAGCACCAAGGAGGTCCTCGACGCGACCCTGATCCACCAGTCCATCACCGGCCTGTACGAAACCCGCATCGACCTCTCCCAGCTCGGCGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
lacZ-alpha lacZ fragment LacZ-alpha fragment of beta-galactosida… 8127..8424 Rev
DNA Sequence
ATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCTGA
Protein Sequence
MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG
ApmR aac(3)IV aminoglycoside 3-N-acetyltransferase ty… 9541..10342 Fwd
DNA Sequence
ATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGCTCATCGGTCAGCTTCTCAACCTTGGGGTTACCCCCGGCGGTGTGCTGCTGGTCCACAGCTCCTTCCGTAGCGTCCGGCCCCTCGAAGATGGGCCACTTGGACTGATCGAGGCCCTGCGTGCTGCGCTGGGTCCGGGAGGGACGCTCGTCATGCCCTCGTGGTCAGGTCTGGACGACGAGCCGTTCGATCCTGCCACGTCGCCCGTTACACCGGACCTTGGAGTTGTCTCTGACACATTCTGGCGCCTGCCAAATGTAAAGCGCAGCGCCCATCCATTTGCCTTTGCGGCAGCGGGGCCACAGGCAGAGCAGATCATCTCTGATCCATTGCCCCTGCCACCTCACTCGCCTGCAAGCCCGGTCGCCCGTGTCCATGAACTCGATGGGCAGGTACTTCTCCTCGGCGTGGGACACGATGCCAACACGACGCTGCATCTTGCCGAGTTGATGGCAAAGGTTCCCTATGGGGTGCCGAGACACTGCACCATTCTTCAGGATGGCAAGTTGGTACGCGTCGATTATCTCGAGAATGACCACTGCTGTGAGCGCTTTGCCTTGGCGGACAGGTGGCTCAAGGAGAAGAGCCTTCAGAAGGAAGGTCCAGTCGGTCATGCCTTTGCTCGGTTGATCCGCTCCCGCGACATTGTGGCGACAGCCCTGGGTCAACTGGGCCGAGATCCGTTGATCTTCCTGCATCCGCCAGAGGCGGGATGCGAAGAATGCGATGCCGCTCGCCAGTCGATTGGC
Protein Sequence
MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRPLEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNVKRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLHLAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGHAFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG
promoter 4
Label Gene Product Position Strand
gapdh promoter — — 7834..8119 Fwd
DNA Sequence
GCTGCTCCTTCGGTCGGACGTGCGTCTACGGGCACCTTACCGCAGCCGTCGGCTGTGCGACACGGACGGATCGGGCGAACTGGCCGATGCTGGGAGAAGCGCGCTGCTGTACGGCGCGCACCGGGTGCGGAGCCCCTCGGCGAGCGGTGTGAAACTTCTGTGAATGGCCTGTTCGGTTGCTTTTTTTATACGGCTGCCAGATAAGGCTTGCAGCATCTGGGCGGCTACCGCTATGATCGGGGCGTTCCTGCAATTCTTAGTGCGAGTATCTGAAAGGGGATACGC
T7 promoter — — 8314..8333 Fwd
DNA Sequence
TAATACGACTCACTATAGG
T3 promoter — — 8380..8399 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter — — 8467..8498 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator — — 8443..8460 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 8512..8534 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori — — 8749..9338 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGGTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator — — 8673..8708 Fwd
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT