| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 6xHis | — | 6xHis affinity tag | 42..60 | Fwd | |
|
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
|
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| thrombin site | — | thrombin recognition and cleavage site | 69..87 | Fwd | |
|
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
|
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| T7 tag (gene 10 leader) | — | leader peptide from bacteriophage T7 ge… | 90..123 | Fwd | |
|
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
|
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| TEV site | — | tobacco etch virus (TEV) protease recog… | 150..171 | Fwd | |
|
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
|
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| BC2 tag | — | nanobody epitope tag corresponding to a… | 216..252 | Fwd | |
|
DNA Sequence
CCGGATCGCAAGGCTGCAGTCAGCCATTGGCAGCAA
Protein Sequence
PDRKAAVSHWQQ
|
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