pDEST17-UbE2E2

15788 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Wade Harper
Article
Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.
Insert
Ubiquitin conjugating enzyme UbE2E2 (UBE2E2 Human)
Tags
His
Available Since
Sept. 28, 2007
Availability
Available

Sequence Information

Length
616 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 36..54 Fwd
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
T7 tag (gene 10 leader) leader peptide from bacteriophage T7 ge… 57..90 Fwd
DNA Sequence
ATGGCTAGCATGACTGGTGGACAGCAAATGGGT
Protein Sequence
MASMTGGQQMG
Xpress(TM) tag Xpress(TM) epitope tag, including an en… 93..117 Fwd
DNA Sequence
GATCTGTACGACGATGACGATAAG
Protein Sequence
DLYDDDDK
protein_bind 1
Label Gene Product Position Strand
attB1 mutant version of attB 144..169 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT