pET41a-MED18

15433 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Joan Conaway, Ronald Conaway
Article
Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-
Insert
MED18 (MED18 Human)
Tags
GST and His
Mutation
N/A
Available Since
Aug. 10, 2007
Availability
Available

Sequence Information

Length
682 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 27..45 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 54..72 Fwd
DNA Sequence
CTGGTGCCACGCGGTAGT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 87..132 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site enterokinase recognition and cleavage s… 162..177 Fwd
DNA Sequence
GATGACGACGACAAG
Protein Sequence
DDDDK