pET41a-MED22

15432 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Joan Conaway, Ronald Conaway
Article
Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-
Insert
MED22 (MED22 Human)
Tags
GST and His
Mutation
N/A
Available Since
Aug. 10, 2007
Availability
Available

Sequence Information

Length
600 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 28..46 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 55..73 Fwd
DNA Sequence
CTGGTGCCACGCGGTAGT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 88..133 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site enterokinase recognition and cleavage s… 163..178 Fwd
DNA Sequence
GATGACGACGACAAG
Protein Sequence
DDDDK