pWLxR5

149490 Synthetic Bacterial Expression

Plasmid Information

Purpose
Broad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.
Depositor
Christopher Reisch
Article
Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629
Insert
LuxR-mRFP
Promoter
PLuxB
Available Since
March 9, 2021
Availability
Available

Sequence Information

Length
6,848 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 3
Label Gene Product Position Strand
RepA repA replication protein of plasmid pSa 950..1922 Fwd
DNA Sequence
ATGCCTAAGAACAACAAAGCCCCCGGCCATCGTATCAACGAGATCATCAAGACGAGCCTCGCGCTCGAAATGGAGGATGCCCGCGAAGCTGGCTTAGTCGGCTACATGGCCCGTTGCCTTGTGCAAGCGACCATGCCCCACACCGACCCCAAGACCAGCTACTTTGAGCGCACCAATGGCATCGTCACCTTGTCGATCATGGGCAAGCCGAGCATCGGCCTGCCCTACGGTTCTATGCCGCGCACCTTGCTTGCTTGGATATGCACCGAGGCCGTGCGAACGAAAGACCCCGTGTTGAACCTTGGCCGGTCGCAATCGGAATTTCTACAAAGGCTCGGAATGCACACCGATGGCCGTTACACGGCCACCCTTCGCAATCAGGCGCAACGCCTGTTTTCATCCATGATTTCGCTTGCCGGCGAGCAAGGCAATGACTTCGGCATTGAGAACGTCGTCATTGCCAAGCGCGCTTTTCTATTCTGGAATCCCAAGCGGCCAGAAGATCGGGCGCTATGGGATAGCACCCTCACCCTCACAGGCGATTTCTTCGAGGAAGTCACCCGCTCACCGGTTCCTATCCGAATCGACTACCTGCATGCCTTGCGGCAGTCTCCGCTTGCGATGGACATTTACACGTGGCTGACCTATCGCGTGTTCCTGTTGCGGGCCAAGGGCCGCCCCTTCGTGCAAATCCCTTGGGTCGCCCTGCAAGCGCAATTCGGCTCATCCTATGGCAGCCGCGCACGCAACTCGCCCGAACTGGACGATAAGGCCCGAGAGCGGGCAGAGCGGGCAGCACTCGCCAGCTTCAAATACAACTTCAAAAAGCGCCTACGCGAAGTGTTGATTGTCTATCCCGAGGCAAGCGACTGCATCGAAGATGACGGCGAATGCCTGCGCATCAAATCCACACGCCTGCATGTCACCCGCGCACCCGGCAAGGGCGCTCGCATCGGCCCCCCTCCGACTTGA
Protein Sequence
MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATMPHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRSQSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNPKRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLRAKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREVLIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT
mRFP1 monomeric derivative of DsRed (Campbell… 5076..5754 Fwd
DNA Sequence
ATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAA
Protein Sequence
MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTGA
GmR aacC1 gentamycin acetyltransferase 6247..6781 Fwd
DNA Sequence
ATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGAGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAA
Protein Sequence
MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIREEVMHFDIDPSTAT
promoter 1
Label Gene Product Position Strand
Pc promoter intI1 (promoter lies within the coding sequence) 6030..6059 Fwd
DNA Sequence
TTGACATAAGCCTGTTCGGTTCGTAAACT
rep_origin 1
Label Gene Product Position Strand
pSa ori 1958..2394 Fwd
DNA Sequence
CCATCCAACAGCCCGCCGTCGAGCGGGCTTTTTTATCCCCGGAAGCCTGTGGATAGAGGGTAGTTATCCACGTGAAACCGCTAATGCCCCGCAAAGCCTTGATTCACGGGGCTTTCCGGCCCGCTCCAAAAACTATCCACGTGAAATCGCTAATCAGGGTACGTGAAATCGCTAATCGGAGTACGTGAAATCGCTAATAAGGTCACGTGAAATCGCTAATCAAAAAGGCACGTGAGAACGCTAATAGCCCTTTCAGATCAACAGCTTGCAAACACCCCTCGCTCCGGCAAGTAGTTACAGCAAGTAGTATGTTCAATTAGCTTTTCAATTATGAATATATATATCAATTATTGGTCGCCCTTGGCTTGTGGACAATGCGCTACGCGCACCGGCTCCGCCCGTGGACAACCGCAAGCGGTTGCCCACCGTCGAGCGC
terminator 1
Label Gene Product Position Strand
his operon terminator 48..106 Fwd
DNA Sequence
TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGA