pM1s3ATScsg-Etag

137946 Synthetic Bacterial Expression

Plasmid Information

Purpose
E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression
Depositor
Neel Joshi
Article
Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
Promoter
pTlpA36
Available Since
April 27, 2021
Availability
Available

Sequence Information

Length
9,938 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 3
Label Gene Product Position Strand
AmpR bla beta-lactamase 408..1269 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCCGAAGATCAGTTGGGTGCACGTGTGGGTTACATCGAACTGGACCTCAACAGCGGTAAGATTCTTGAGAGTTTTCGCCCCGAAGAACGTTTCCCAATGATGAGCACTTTTAAAGTTCTGCTCTGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGACGGCATGACAGTACGCGAATTATGCAGCGCTGCCATAACCATGAGTGATAACACGGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTTACCGCTTTTTTGCACAACATGGGTGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCTATGGCAACAACGTTGCGCAAACTCTTAACTGGCGAACTTCTTACTCTCGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCCCGCGGTATTATTGCAGCCCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGCCAGGCAACTATGGACGAACGTAATCGCCAGATCGCTGAGATAGGTGCCTCCCTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
S-Tag affinity and epitope tag derived from p… 6333..6378 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCGAAATTTGAACGCCAGCACATGGACTCG
Protein Sequence
KETAAAKFERQHMDS
6xHis 6xHis affinity tag 6648..6666 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
lacI promoter lacI 5100..5178 Fwd
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
terminator 4
Label Gene Product Position Strand
T3Te terminator 231..261 Rev
DNA Sequence
GGCTCACCTTCACGGGTGGGCCTTTCTTCG
T3Te terminator 1398..1428 Rev
DNA Sequence
GGCTCACCTTCACGGGTGGGCCTTTCTTCG
T7 terminator 6429..6477 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
T7 terminator 6732..6780 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG