pET30 SUMO-1 G97

13381 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Mary Dasso
Article
Saitoh et al Curr Biol. 1998 Jan 15. 8(2):121-4.
Insert
SUMO-1, G97 (SUMO1 Human)
Tags
His
Mutation
G97 mutant: lacks 4 amino acids from the C-termin…
Available Since
Nov. 16, 2006
Availability
Available

Sequence Information

Length
426 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 4
Label Gene Product Position Strand
6xHis 6xHis affinity tag 12..30 Fwd
DNA Sequence
CACCATCATCATCATCAT
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 39..57 Fwd
DNA Sequence
CTGGTGCCACGCGGTTCT
Protein Sequence
LVPRGS
S-Tag affinity and epitope tag derived from p… 63..108 Fwd
DNA Sequence
AAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGC
Protein Sequence
KETAAAKFERQHMDS
enterokinase site enterokinase recognition and cleavage s… 123..138 Fwd
DNA Sequence
GACGACGACGACAAG
Protein Sequence
DDDDK