pWS926

123052 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
LexA bacterial repressor
Depositor
Tom Ellis
Article
Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do
Insert
LexA-NLS [3a]
Available Since
April 5, 2019
Availability
Available

Sequence Information

Length
2,313 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 3
Label Gene Product Position Strand
LexA lexA E. coli DNA-binding protein that repres… 12..618 Fwd
DNA Sequence
ATGAAGGCTTTAACTGCAAGACAACAGGAAGTTTTTGATTTGATAAGAGATCATATATCTCAAACTGGAATGCCACCAACAAGAGCTGAAATAGCTCAAAGATTAGGATTTAGATCTCCAAACGCTGCAGAAGAACATTTGAAAGCTTTGGCAAGGAAAGGTGTCATCGAAATTGTCTCTGGTGCTTCAAGAGGTATTAGACTATTACAAGAAGAGGAAGAAGGATTGCCATTGGTTGGTAGAGTTGCTGCTGGTGAACCATTATTAGCACAGCAACATATTGAGGGTCATTATCAAGTAGATCCATCTTTATTCAAACCAAATGCTGATTTTTTGTTGAGAGTAAGTGGTATGTCTATGAAAGATATTGGTATCATGGATGGTGACTTATTGGCTGTTCATAAAACTCAAGATGTTAGAAACGGTCAAGTTGTCGTTGCCAGAATTGATGATGAAGTCACTGTTAAAAGATTGAAGAAACAAGGTAATAAAGTGGAATTGTTACCAGAAAATTCTGAATTTAAGCCAATTGTTGTTGATTTAAGGCAACAATCTTTTACTATTGAAGGTTTAGCTGTCGGTGTTATCAGAAATGGTGATTGGTTA
Protein Sequence
MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL
SV40 NLS nuclear localization signal of SV40 (si… 633..654 Fwd
DNA Sequence
CCAAAAAAAAAGAGAAAAGTT
Protein Sequence
PKKKRKV
CmR cat chloramphenicol acetyltransferase 784..1444 Rev
DNA Sequence
ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTTTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA
Protein Sequence
MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA
rep_origin 1
Label Gene Product Position Strand
ori 1625..2214 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
lambda t0 terminator 679..714 Rev
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT