pTHSSe_50

109248 Synthetic Bacterial Expression

Plasmid Information

Purpose
incW BbsI cloning vector
Depositor
Christopher Voigt
Article
Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41
Insert
sfGFP
Promoter
PJ23105
Available Since
July 6, 2018
Availability
Available

Sequence Information

Length
6,101 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
RepA repA replication protein of plasmid pSa 734..1706 Rev
DNA Sequence
ATGCCTAAGAACAACAAAGCCCCCGGCCATCGTATCAACGAGATCATCAAGACGAGCCTCGCGCTCGAAATGGAGGATGCCCGCGAAGCTGGCTTAGTCGGCTACATGGCCCGTTGCCTTGTGCAAGCGACCATGCCCCACACCGACCCCAAGACCAGCTACTTTGAGCGCACCAATGGCATCGTCACCTTGTCGATCATGGGCAAGCCGAGCATCGGCCTGCCCTACGGTTCTATGCCGCGCACCTTGCTTGCTTGGATATGCACCGAGGCCGTGCGAACGAAAGACCCCGTGTTGAACCTTGGCCGGTCGCAATCGGAATTTCTACAAAGGCTCGGAATGCACACCGATGGCCGTTACACGGCCACCCTTCGCAATCAGGCGCAACGCCTGTTTTCATCCATGATTTCGCTTGCCGGCGAGCAAGGCAATGACTTCGGCATTGAGAACGTCGTCATTGCCAAGCGCGCTTTTCTATTCTGGAATCCCAAGCGGCCAGAAGATCGGGCGCTATGGGATAGCACCCTCACCCTCACAGGCGATTTCTTCGAGGAAGTCACCCGCTCACCGGTTCCTATCCGAATCGACTACCTGCATGCCTTGCGGCAGTCTCCGCTTGCGATGGACATTTACACGTGGCTGACCTATCGCGTGTTCCTGTTGCGGGCCAAGGGCCGCCCCTTCGTGCAAATCCCTTGGGTCGCCCTGCAAGCGCAATTCGGCTCATCCTATGGCAGCCGCGCACGCAACTCGCCCGAACTGGACGATAAGGCCCGAGAGCGGGCAGAGCGGGCAGCACTCGCCAGCTTCAAATACAACTTCAAAAAGCGCCTACGCGAAGTGTTGATTGTCTATCCCGAGGCAAGCGACTGCATCGAAGATGACGGCGAATGCCTGCGCATCAAATCCACACGCCTGCATGTCACCCGCGCACCCGGCAAGGGCGCTCGCATCGGCCCCCCTCCGACTTGA
Protein Sequence
MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATMPHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRSQSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNPKRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLRAKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREVLIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT
superfolder GFP — GFP variant that folds robustly even wh… 2778..3495 Rev
DNA Sequence
ATGAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCCGTGGAGAGGGTGAAGGTGATGCTACAAACGGAAAACTCACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCGTGGCCAACACTTGTCACTACTCTGACCTATGGTGTTCAATGCTTTTCCCGTTATCCGGATCACATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAACGCACTATATCTTTCAAAGATGACGGGACCTACAAGACGCGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATCGTATCGAGTTAAAGGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAACTCGAGTACAACTTTAACTCACACAATGTATACATCACGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATTCGCCACAACGTTGAAGATGGTTCCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCGACACAATCTGTCCTTTCGAAAGATCCCAACGAAAAGCGTGACCACATGGTCCTTCTTGAGTTTGTAACTGCTGCTGGGATTACACATGGCATGGATGAGCTCTACAAATAA
Protein Sequence
MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
superfolder GFP — GFP variant that folds robustly even wh… 2781..3495 Rev
DNA Sequence
ATGAGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCCGTGGAGAGGGTGAAGGTGATGCTACAAACGGAAAACTCACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCGTGGCCAACACTTGTCACTACTCTGACCTATGGTGTTCAATGCTTTTCCCGTTATCCGGATCACATGAAACGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAACGCACTATATCTTTCAAAGATGACGGGACCTACAAGACGCGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATCGTATCGAGTTAAAGGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAACTCGAGTACAACTTTAACTCACACAATGTATACATCACGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAATTCGCCACAACGTTGAAGATGGTTCCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCGACACAATCTGTCCTTTCGAAAGATCCCAACGAAAAGCGTGACCACATGGTCCTTCTTGAGTTTGTAACTGCTGCTGGGATTACACATGGCATGGATGAGCTCTACAAA
Protein Sequence
MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
AmpR bla beta-lactamase 3911..4772 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTATATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 2
Label Gene Product Position Strand
BBa_J23105 — — 3594..3629 Rev
DNA Sequence
TTTACGGCTAGCTCAGTCCTAGGTACTATGCTAGC
AmpR promoter bla — 3806..3911 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
pSa ori — — 262..698 Fwd
DNA Sequence
GCGCTCGACGGTGGGCAACCGCTTGCGGTTGTCCACGGGCGGAGCCGGTGCGCGTAGCGCATTGTCCACAAGCCAAGGGCGACCAATAATTGATATATATATTCATAATTGAAAAGCTAATTGAACATACTACTTGCTGTAACTACTTGCCGGAGCGAGGGGTGTTTGCAAGCTGTTGATCTGAAAGGGCTATTAGCGTTCTCACGTGCCTTTTTGATTAGCGATTTCACGTGACCTTATTAGCGATTTCACGTACTCCGATTAGCGATTTCACGTACCCTGATTAGCGATTTCACGTGGATAGTTTTTGGAGCGGGCCGGAAAGCCCCGTGAATCAAGGCTTTGCGGGGCATTAGCGGTTTCACGTGGATAACTACCCTCTATCCACAGGCTTCCGGGGATAAAAAAGCCCGCTCGACGGCGGGCTGTTGGATGG
terminator 4
Label Gene Product Position Strand
his operon terminator — — 2550..2608 Rev
DNA Sequence
TCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGA
T7Te terminator — — 2647..2675 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
rrnB T1 terminator Escherichia coli rrnB — 2690..2762 Rev
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
bacterial terminator — — 3673..3717 Fwd
DNA Sequence
GAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAT