p15aC-4D1D-5

107866 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of PIS, PI4K and PI4P5K
Depositor
Sanford Simon
Article
Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:
Tags
myc
Promoter
proD and proD (in operon)
Available Since
April 8, 2019
Availability
Available

Sequence Information

Length
7,316 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 4
Label Gene Product Position Strand
Myc Myc (human c-Myc proto-oncogene) epitop… 381..411 Fwd
DNA Sequence
GAACAGAAATTGATTAGCGAAGAGGATTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 3948..3978 Fwd
DNA Sequence
GAGCAGAAATTGATTAGCGAGGAAGATTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 4688..4718 Fwd
DNA Sequence
GAACAAAAGTTGATCAGCGAAGAAGACTTG
Protein Sequence
EQKLISEEDL
CmR cat chloramphenicol acetyltransferase 6656..7316 Rev
DNA Sequence
ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAGTTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA
Protein Sequence
MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA
RBS 3
Label Gene Product Position Strand
Shine-Dalgarno sequence 364..373 Fwd
DNA Sequence
TAAGGAGGT
Shine-Dalgarno sequence 3932..3941 Fwd
DNA Sequence
TAAGGAGGT
Shine-Dalgarno sequence 4668..4677 Fwd
DNA Sequence
TAAGGAGGT
promoter 1
Label Gene Product Position Strand
AmpR promoter bla 0..105 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
rep_origin 1
Label Gene Product Position Strand
p15A ori 3111..3657 Rev
DNA Sequence
TTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAA