lac operator (symmetric)

Feature Information

Note

The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). The symmetric lac operator was optimized for tight binding of lac repressor.

Type

protein_bind

Found in 79 Plasmids (showing 1–50)

Plasmid	Species	Position	Strand
pAJM.715 108516	Synthetic	2871..2891	Fwd
pAJM.336 108528	Synthetic	4282..4302	Fwd
pTHSSe_37 109237	Synthetic	36..56	Fwd
pTHSSe_39 109238	Synthetic	32..52	Fwd
pTHSSe_40 109239	Synthetic	36..56	Fwd
pDNA2.0 6H-FLAG-TEV-KRAS G12C 111848	Homo sapiens	1503..1523	Fwd
pDNA2.0 6H-TEV-KRAS 111849	Homo sapiens	1504..1524	Fwd
pDNA2.0 6H-TEV-KRAS G12V 111850	Homo sapiens	1504..1524	Fwd
pDNA2.0 6H-TEV-KRAS G13D 111851	Homo sapiens	1499..1519	Fwd
pBP-Pspac 112778	Synthetic	2172..2192	Fwd
pJUMP19-Ptac_P 127004	Synthetic	121..141	Fwd
pVB004 127188	Synthetic	1948..1968	Fwd
SpyCatcher003-sfGFP 133449	Synthetic	805..825	Fwd
pMGA-ptac-sfGFP 139934	Synthetic	1426..1446	Fwd
pSW039 140035	Synthetic	400..420	Fwd
pOP/SSAT 140291	Homo sapiens	5746..5766	Fwd
pOP/SSAT 140291	Homo sapiens	6125..6145	Fwd
pVB006 140327	Synthetic	188..208	Fwd
pAKW.119-R5-TEV-SUMO-His 154050	Synthetic	34..54	Fwd
KRAS-A146T 154426	Homo sapiens	659..679	Fwd
KRAS-V14I 154427	Homo sapiens	334..354	Fwd
KRAS-P34R 158527	Homo sapiens	654..674	Fwd
pDNA2.0-6H-TEV-KRAS G12D 159439	Homo sapiens	708..728	Fwd
pSB208 164123	Synthetic	1173..1193	Fwd
pB-01 166924	Synthetic	136..156	Fwd
pH-01 166936	Synthetic	1519..1539	Fwd
pPtac 166937	Synthetic	2396..2416	Fwd
pTHS 166938	Synthetic	1179..1199	Fwd
pTHS 166938	Synthetic	2395..2415	Fwd
pSTAR 166939	Synthetic	1311..1331	Fwd
pSTAR 166939	Synthetic	2581..2601	Fwd
pSTAR2 166940	Synthetic	1311..1331	Fwd
pSTAR2 166940	Synthetic	2601..2621	Fwd
pDC 166941	Synthetic	1311..1331	Fwd
pDC 166941	Synthetic	2490..2510	Fwd
pYY_S1_400 170470	Synthetic	2591..2611	Fwd
pYY_S2_400 170471	Synthetic	4307..4327	Fwd
pRS_SN_400 170472	Synthetic	2591..2611	Fwd
pRS_2S_400 170478	Synthetic	2952..2972	Fwd
pJ404-DogCatcher-sfGFP 171930	Synthetic	1421..1441	Fwd
KTK_247 180529	Synthetic	1164..1184	Fwd
SpyTag-sfGFP 184226	Synthetic	1657..1677	Fwd
pAJT237-Ptac 202297	Synthetic	848..868	Fwd
pAJT249-psrA 202304	Synthetic	2083..2103	Fwd
pAJT251-bm3R1 202306	Synthetic	784..804	Fwd
pAJT252-amtR 202307	Synthetic	892..912	Fwd
pAJT254-litR 202308	Synthetic	861..881	Fwd
pAJT255-ameRS 202309	Synthetic	892..912	Fwd
pBP-P_lacI-Ptac-lacO 204072	Synthetic	2392..2412	Fwd
pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO 204079	Synthetic	5378..5398	Fwd

DNA Sequence Variants

Sequence context may differ across plasmids due to position and neighboring features.

pAJM.715 108516

2871..2891 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pAJM.336 108528

4282..4302 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pTHSSe_37 109237

36..56 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pTHSSe_39 109238

32..52 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pTHSSe_40 109239

36..56 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pDNA2.0 6H-FLAG-TEV-KRAS G12C 111848

1503..1523 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pDNA2.0 6H-TEV-KRAS 111849

1504..1524 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pDNA2.0 6H-TEV-KRAS G12V 111850

1504..1524 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pDNA2.0 6H-TEV-KRAS G13D 111851

1499..1519 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pBP-Pspac 112778

2172..2192 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pJUMP19-Ptac_P 127004

121..141 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pVB004 127188

1948..1968 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

SpyCatcher003-sfGFP 133449

805..825 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pMGA-ptac-sfGFP 139934

1426..1446 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pSW039 140035

400..420 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pOP/SSAT 140291

5746..5766 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pOP/SSAT 140291

6125..6145 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pVB006 140327

188..208 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pAKW.119-R5-TEV-SUMO-His 154050

34..54 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

KRAS-A146T 154426

659..679 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

KRAS-V14I 154427

334..354 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

KRAS-P34R 158527

654..674 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pDNA2.0-6H-TEV-KRAS G12D 159439

708..728 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pSB208 164123

1173..1193 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pB-01 166924

136..156 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pH-01 166936

1519..1539 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pPtac 166937

2396..2416 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pTHS 166938

1179..1199 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pTHS 166938

2395..2415 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pSTAR 166939

1311..1331 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pSTAR 166939

2581..2601 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pSTAR2 166940

1311..1331 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pSTAR2 166940

2601..2621 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pDC 166941

1311..1331 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pDC 166941

2490..2510 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pYY_S1_400 170470

2591..2611 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pYY_S2_400 170471

4307..4327 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pRS_SN_400 170472

2591..2611 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pRS_2S_400 170478

2952..2972 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pJ404-DogCatcher-sfGFP 171930

1421..1441 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

KTK_247 180529

1164..1184 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

SpyTag-sfGFP 184226

1657..1677 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pAJT237-Ptac 202297

848..868 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pAJT249-psrA 202304

2083..2103 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pAJT251-bm3R1 202306

784..804 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pAJT252-amtR 202307

892..912 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pAJT254-litR 202308

861..881 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pAJT255-ameRS 202309

892..912 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pBP-P_lacI-Ptac-lacO 204072

2392..2412 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT

pBP-P_TALEsp1_PUPsp1-lacI_Ptac-lacO 204079

5378..5398 (Fwd)

DNA Sequence (20 bp)

AATTGTGAGCGCTCACAATT