| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| Myc | — | Myc (human c-Myc proto-oncogene) epitop… | 680..710 | Rev | |
|
DNA Sequence
GAACAAAAACTGATATCAGAAGAGGATCTG
Protein Sequence
EQKLISEEDL
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| TEV site | — | tobacco etch virus (TEV) protease recog… | 710..731 | Rev | |
|
DNA Sequence
GAGAACCTCTACTTCCAAGGA
Protein Sequence
ENLYFQG
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| 3xFLAG | — | three tandem FLAG(R) epitope tags, foll… | 731..797 | Rev | |
|
DNA Sequence
GACTACAAAGACCATGACGGTGATTATAAAGATCATGATATCGATTACAAGGATGACGATGACAAG
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
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| 3xFLAG | — | three tandem FLAG(R) epitope tags, foll… | 1484..1550 | Rev | |
|
DNA Sequence
GACTACAAGGACCACGACGGAGACTACAAGGACCACGACATCGACTACAAGGACGACGACGACAAG
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
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| TEV site | — | tobacco etch virus (TEV) protease recog… | 1550..1571 | Rev | |
|
DNA Sequence
GAAAATTTATATTTTCAGGGT
Protein Sequence
ENLYFQG
|
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| Myc | — | Myc (human c-Myc proto-oncogene) epitop… | 1571..1601 | Rev | |
|
DNA Sequence
GAGCAAAAGCTCATCTCCGAGGAGGACCTC
Protein Sequence
EQKLISEEDL
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| AmpR | bla | beta-lactamase | 2222..3083 | Fwd | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
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| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac promoter | — | — | 554..585 | Fwd | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
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| AmpR promoter | bla | — | 2117..2222 | Fwd | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
|
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| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| CAP binding site | — | — | 518..540 | Fwd | |
|
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
|
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| lac operator | — | — | 592..609 | Fwd | |
|
DNA Sequence
TTGTGAGCGGATAACAA
|
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| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 0..3611 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
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