pMV762-Peredox-mCherry

90217 Synthetic Bacterial Expression

Plasmid Information

Purpose
Fluorescent reporter for NADH:NAD+ ratios in Mycobacteria.
Depositor
Ashwani Kumar
Article
Bhat et al Front Cell Infect Microbiol. 2016 Nov 8;
Insert
Codon Optimized Peredox-mCherry
Promoter
groEL
Available Since
June 26, 2017
Availability
Available

Sequence Information

Length
7,316 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

rep_origin 1
Label Gene Product Position Strand
ori — — 4642..5231 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAGGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
rrnB T1 terminator Escherichia coli rrnB — 3223..3270 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTAT