pLZ12Km2-P23R:TA:Xen5

88902 Synthetic Bacterial Expression

Plasmid Information

Purpose
Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression
Depositor
Thomas Proft
Article
Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2
Insert
toxin-antitoxin + LuxABCDE
Available Since
April 5, 2017
Availability
Available

Sequence Information

Length
13,409 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 7
Label Gene Product Position Strand
Factor Xa site Factor Xa recognition and cleavage site 3550..3562 Rev
DNA Sequence
ATAGAAGGACGC
Protein Sequence
IEGR
KanR aph(3')-Ia aminoglycoside phosphotransferase 5202..5997 Rev
DNA Sequence
ATGGCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCGCTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGGTGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAAGGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGAAGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCTGGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAGTATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTGCATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATAGCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTGGCCGATGTGGATTGCGAAAACTGGGAAGAAGACACTCCATTTAAAGATCCGCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTGTCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGCAAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTGGTATGACATTGCCTTCTGCGTCCGGTCGATCAGGGAGGATATCGGGGAAGAACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGGGAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAG
Protein Sequence
MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF
LuxA luxA LuxA luciferase subunit 7293..8376 Fwd
DNA Sequence
ATGAAATTTGGAAACTTTTTGCTTACATACCAACCTCCCCAATTTTCTCAAACAGAGGTAATGAAACGTTTGGTTAAATTAGGTCGCATCTCTGAGGAGTGTGGTTTTGATACCGTATGGTTACTGGAGCATCATTTCACGGAGTTTGGTTTGCTTGGTAACCCTTATGTCGCTGCTGCATATTTACTTGGCGCGACTAAAAAATTGAATGTAGGAACTGCCGCTATTGTTCTTCCCACAGCCCATCCAGTACGCCAACTTGAAGATGTGAATTTATTGGATCAAATGTCAAAAGGACGATTTCGGTTTGGTATTTGCCGAGGGCTTTACAACAAGGACTTTCGCGTATTCGGCACAGATATGAATAACAGTCGCGCCTTAGCGGAATGCTGGTACGGGCTGATAAAGAATGGCATGACAGAGGGATATATGGAAGCTGATAATGAACATATCAAGTTCCATAAGGTAAAAGTAAACCCCGCGGCGTATAGCAGAGGTGGCGCACCGGTTTATGTGGTGGCTGAATCAGCTTCGACGACTGAGTGGGCTGCTCAATTTGGCCTACCGATGATATTAAGTTGGATTATAAATACTAACGAAAAGAAAGCACAACTTGAGCTTTATAATGAAGTGGCTCAAGAATATGGGCACGATATTCATAATATCGACCATTGCTTATCATATATAACATCTGTAGATCATGACTCAATTAAAGCGAAAGAGATTTGCCGGAAATTTCTGGGGCATTGGTATGATTCTTATGTGAATGCTACGACTATTTTTGATGATTCAGACCAAACAAGAGGTTATGATTTCAATAAAGGGCAGTGGCGTGACTTTGTATTAAAAGGACATAAAGATACTAATCGCCGTATTGATTACAGTTACGAAATCAATCCCGTGGGAACGCCGCAGGAATGTATTGACATAATTCAAAAAGACATTGATGCTACAGGAATATCAAATATTTGTTGTGGATTTGAAGCTAATGGAACAGTAGACGAAATTATTGCTTCCATGAAGCTCTTCCAGTCTGATGTCATGCCATTTCTTAAAGAAAAACAACGTTCGCTATTATATTAA
Protein Sequence
MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHHFTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFGICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAYSRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNIDHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFVLKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASMKLFQSDVMPFLKEKQRSLLY
LuxB luxB LuxB luciferase subunit 8396..9380 Fwd
DNA Sequence
ATGAAATTTGGATTGTTCTTCCTTAACTTCATCAATTCAACAACTGTTCAAGAACAAAGTATAGTTCGCATGCAGGAAATAACGGAGTATGTTGATAAGTTGAATTTTGAACAGATTTTAGTGTATGAAAATCATTTTTCAGATAATGGTGTTGTCGGCGCTCCTCTGACTGTTTCTGGTTTTCTGCTCGGTTTAACAGAGAAAATTAAAATTGGTTCATTAAATCACATCATTACAACTCATCATCCTGTCCGCATAGCGGAGGAAGCTTGCTTATTGGATCAGTTAAGTGAAGGGAGATTTATTTTAGGGTTTAGTGATTGCGAAAAAAAAGATGAAATGCATTTTTTTAATCGCCCGGTTGAATATCAACAGCAACTATTTGAAGAGTGTTATGAAATCATTAACGATGCTTTAACAACAGGCTATTGTAATCCAGATAACGATTTTTATAGCTTCCCTAAAATATCTGTAAATCCCCATGCTTATACGCCAGGCGGACCTCGGAAATATGTAACAGCAACCAGTCATCATATTGTTGAGTGGGCGGCCAAAAAAGGTATTCCTCTCATCTTTAAGTGGGATGATTCTAATGATGTTAGATATGAATATGCTGAAAGATATAAAGCCGTTGCGGATAAATATGACGTTGACCTATCAGAGATAGACCATCAGTTAATGATATTAGTTAACTATAACGAAGATAGTAATAAAGCTAAACAAGAGACGCGTGCATTTATTAGTGATTATGTTCTTGAAATGCACCCTAATGAAAATTTCGAAAATAAACTTGAAGAAATAATTGCAGAAAACGCTGTCGGAAATTATACGGAGTGTATAACTGCGGCTAAGTTGGCAATTGAAAAGTGTGGTGCGAAAAGTGTATTGCTGTCCTTTGAACCAATGAATGATTTGATGAGCCAAAAAAATGTAATCAATATTGTTGATGATAATATTAAGAAGTACCACATGGAATATACCTAA
Protein Sequence
MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHFSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGFSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYTPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEIDHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITAAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT
LuxC luxC LuxC fatty acid reductase 9399..10842 Fwd
DNA Sequence
ATGACTAAAAAAATTTCATTCATTATTAACGGCCAGGTTGAAATCTTTCCCGAAGGTGATGATTTAGTGCAATCCATTAATTTTGGTGATAATAGTGTTTACCTGCCAATATTGAATGACTCTCATGTAAAAAACATTATTGATTGTAATGGAAATAACGAATTACGGTTGCATAACATTGTCAATTTTCTCTATACGGTAGGGCAAAGATGGAAAAATGAAGAATACTCAAGACGCAGGACATACATTCGTGACTTAAAAAAATATATGGGATATTCAGAAGAAATGGCTAAGCTAGAGGCCAATTGGATATCTATGATTTTATGTTCTAAAGGCGGCCTTTATGATGTTGTAGAAAATGAACTTGGTTCTCGCCATATCATGGATGAATGGCTACCTCAGGATGAAAGTTATGTTCGGGCTTTTCCGAAAGGTAAATCTGTACATCTGTTGGCAGGTAATGTTCCACTATCTGGGATCATGTCTATATTACGCGCAATTTTAACTAGGAATCAGTGTATTATAAAAACATCGTCAACCGATCCTTTTACCGCTAATGCATTAGCGTTAAGTTTTATTGATGTAGACCCCAATCATCCGATAACGCGCTCTTTATCTGTTATATATTGGCCCCACCAAGGTGATACATCACTCGCAAAAGAAATTATGCAACATGCGGATGTTATTGTCGCTTGGGGAGGGCCAGATGCGATTAATTGGGCGGTAGAGCATGCGCCATCTTATGCTGATGTGATTAAATTTGGTTCTAAAAAGAGTCTTTGCATTATCGATAATCCTGTTGATTTGACGTCCGCAGCGACAGGTGCGGCTCATGATGTTTGTTTTTACGATCAGCGAGCTTGTTTTTCTGCCCAAAACATATATTACATGGGAAATCATTATGAGGAATTTAAGTTAGCGTTGATAGAAAAACTTAATCTATATGCGCATATATTACCGAATGCCAAAAAAGATTTTGATGAAAAGGCGGCCTATTCTTTAGTTCAAAAAGAAAGCTTGTTTGCTGGATTAAAAGTAGAGGTGGATATTCATCAACGTTGGATGATTATTGAGTCAAATGCAGGTGTGGAATTTAATCAACCACTTGGCAGATGTGTGTACCTTCATCACGTCGATAATATTGAGCAAATATTGCCTTATGTTCAAAAAAATAAGACGCAAACCATATCTATTTTTCCTTGGGAGTCATCATTTAAATATCGAGATGCGTTAGCATTAAAAGGTGCGGAAAGGATTGTAGAAGCAGGAATGAATAACATATTTCGAGTTGGTGGATCTCATGACGGAATGAGACCGTTGCAACGATTAGTGACATATATTTCTCATGAAAGGCCATCTAACTATACGGCTAAGAATGTTGCGGTTGAAATAGAACAGACTCGATTCCTGGAAGAAGATAAGTTCCTTGTATTTGTCCCATAA
Protein Sequence
MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKNIIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWISMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSILRAILTRNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEIMQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVCFYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESLFAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIFPWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAKNVAVEIEQTRFLEEDKFLVFVP
LuxD luxD LuxD acyltransferase 10861..11785 Fwd
DNA Sequence
ATGGAAAATGAATCAAAATATAAAACCATCGACCACGTTATTTGTGTTGAAGGAAATAAAAAAATTCATGTTTGGGAAACGCTGCCAGAAGAAAACAGCCCAAAGAGAAAGAATGCCATTATTATTGCGTCTGGTTTTGCCCGCAGGATGGATCATTTTGCTGGTCTGGCGGAATATTTATCGCGGAATGGATTTCATGTGATCCGCTATGATTCGCTTCACCACGTTGGATTGAGTTCAGGGACAATTGATGAATTTACAATGTCTATAGGAAAGCAGAGCTTGTTAGCAGTGGTTGATTGGTTAACTACACGAAAAATAAATAACTTCGGTATGTTGGCTTCAAGCTTATCTGCGCGGATAGCTTATGCAAGCCTATCTGAAATCAATGCTTCGTTTTTAATCACCGCAGTCGGTGTTGTTAACTTAAGATATTCTCTTGAAAGAGCTTTAGGGTTTGATTATCTCAGTCTACCCATTAATGAATTGCCGAATAATCTAGATTTTGAAGGCCATAAATTGGGTGCTGAAGTCTTTGCGAGAGATTGTCTTGATTTTGGTTGGGAAGATTTAGCTTCTACAATTAATAACATGATGTATCTTGATATACCGTTTATTGCTTTTACTGCAAATAACGATAATTGGGTCAAGCAAGATGAAGTTATCACATTGTTATCAAATATTCGTAGTAATCGATGCAAGATATATTCTTTGTTAGGAAGTTCGCATGACTTGAGTGAAAATTTAGTGGTCCTGCGCAATTTTTATCAATCGGTTACGAAAGCCGCTATCGCGATGGATAATGATCATCTGGATATTGATGTTGATATTACTGAACCGTCATTTGAACATTTAACTATTGCGACAGTCAATGAACGCCGAATGAGAATTGAGATTGAAAATCAAGCAATTTCTCTGTCTTAA
Protein Sequence
MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASGFARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTTRKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINELPNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVITLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPSFEHLTIATVNERRMRIEIENQAISLS
LuxE luxE LuxE 11804..12917 Fwd
DNA Sequence
ATGACTTCATATGTTGATAAACAAGAAATTACAGCAAGCTCAGAAATTGATGATTTGATTTTTTCGAGCGATCCATTAGTGTGGTCTTACGACGAGCAGGAAAAAATCAGAAAGAAACTTGTGCTTGATGCATTTCGTAATCATTATAAACATTGTCGAGAATATCGTCACTACTGTCAGGCACACAAAGTAGATGACAATATTACGGAAATTGATGACATACCTGTATTCCCAACATCGGTTTTTAAGTTTACTCGCTTATTAACTTCTCAGGAAAACGAGATTGAAAGTTGGTTTACCAGTAGCGGCACGAATGGTTTAAAAAGTCAGGTGGCGCGTGACAGATTAAGTATTGAGAGACTCTTAGGCTCTGTGAGTTATGGCATGAAATATGTTGGTAGTTGGTTTGATCATCAAATAGAATTAGTCAATTTGGGACCAGATAGATTTAATGCTCATAATATTTGGTTTAAATATGTTATGAGTTTGGTGGAATTGTTATATCCTACGACATTTACCGTAACAGAAGAACGAATAGATTTTGTTAAAACATTGAATAGTCTTGAACGAATAAAAAATCAAGGGAAAGATCTTTGTCTTATTGGTTCGCCATACTTTATTTATTTACTCTGCCATTATATGAAAGATAAAAAAATCTCATTTTCTGGAGATAAAAGCCTTTATATCATAACCGGAGGCGGCTGGAAAAGTTACGAAAAAGAATCTCTGAAACGTGATGATTTCAATCATCTTTTATTTGATACTTTCAATCTCAGTGATATTAGTCAGATCCGAGATATATTTAATCAAGTTGAACTCAACACTTGTTTCTTTGAGGATGAAATGCAGCGTAAACATGTTCCGCCGTGGGTATATGCGCGAGCGCTTGATCCTGAAACGTTGAAACCTGTACCTGATGGAACGCCGGGGTTGATGAGTTATATGGATGCGTCAGCAACCAGTTATCCAGCATTTATTGTTACCGATGATGTCGGGATAATTAGCAGAGAATATGGTAAGTATCCCGGCGTGCTCGTTGAAATTTTACGTCGCGTCAATACGAGGACGCAGAAAGGGTGTGCTTTAAGCTTAACCGAAGCGTTTGATAGTTGA
Protein Sequence
MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAFRNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGTNGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSLVELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFSGDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDEMQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGKYPGVLVEILRRVNTRTQKGCALSLTEAFDS
promoter 1
Label Gene Product Position Strand
T7 promoter 7049..7068 Fwd
DNA Sequence
TAATACGACTCACTATAGG