pFC334

87846 Synthetic Bacterial Expression

Plasmid Information

Purpose
AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
Depositor
Uffe Mortensen
Article
Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
Tags
SV40 NLS
Promoter
A. nidulans gpdA promoter with Hammerhead ribozym…
Available Since
May 9, 2017
Availability
Available

Sequence Information

Length
17,327 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
AmpR bla beta-lactamase 0..17327 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGTAGCGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 8954..13058 Fwd
DNA Sequence
ATGGACAAGAAGTATAGCATCGGGCTGGACATTGGAACGAACTCGGTTGGTTGGGCTGTGATTACGGACGAATACAAGGTGCCATCCAAGAAGTTTAAGGTCCTGGGAAACACCGACCGTCACTCAATCAAGAAGAATCTCATTGGAGCCCTGCTCTTCGATAGTGGGGAGACCGCCGAAGCTACTCGACTGAAGCGAACGGCTCGCCGGCGTTATACACGACGCAAGAATCGCATCTGCTACCTCCAGGAGATTTTCAGCAACGAAATGGCTAAGGTTGATGACTCATTCTTTCATCGACTCGAAGAAAGTTTCTTGGTCGAGGAGGATAAGAAGCACGAGCGCCATCCGATCTTTGGTAACATTGTGGATGAGGTTGCCTATCACGAAAAGTACCCAACTATCTATCATCTTCGTAAGAAGCTGGTCGATAGCACGGACAAGGCTGATTTGCGACTTATCTACCTGGCACTCGCGCACATGATTAAGTTCCGCGGCCATTTTCTTATCGAGGGTGACCTGAACCCCGATAATTCTGACGTTGATAAGCTCTTCATCCAGTTGGTCCAAACCTACAATCAGCTGTTTGAGGAAAACCCTATTAATGCATCTGGCGTGGACGCCAAGGCTATCCTTTCGGCGCGCCTGTCTAAGTCGCGGCGTTTGGAGAACCTTATCGCACAACTCCCCGGCGAAAAGAAGAACGGCCTCTTCGGTAATTTGATTGCGTTGTCACTTGGTCTGACTCCTAACTTCAAGAGTAATTTTGACCTGGCAGAGGATGCGAAGCTCCAGTTGTCTAAGGATACGTATGATGACGATCTCGACAACTTGCTTGCCCAAATCGGTGACCAGTACGCTGATCTTTTCCTGGCCGCTAAGAATCTCTCAGATGCAATCCTGCTCAGTGACATTTTGCGGGTCAACACCGAGATTACTAAGGCCCCCCTGTCAGCTAGTATGATCAAGCGGTATGATGAGCACCATCAGGACCTCACCTTGCTTAAGGCCCTCGTGCGTCAGCAATTGCCTGAGAAGTATAAGGAAATCTTCTTTGACCAATCCAAGAACGGATACGCAGGGTATATTGATGGCGGTGCGAGCCAGGAGGAATTCTACAAGTTTATCAAGCCGATTTTGGAGAAGATGGACGGCACTGAGGAACTGCTCGTCAAGCTGAATCGCGAAGATTTGCTTCGTAAGCAACGAACGTTCGACAACGGCTCCATCCCGCACCAGATTCATCTGGGCGAGCTCCACGCCATCCTTCGACGCCAGGAAGATTTCTACCCATTTCTGAAGGACAACCGTGAGAAGATCGAAAAGATTCTTACATTCCGAATCCCCTACTATGTGGGACCTTTGGCCCGTGGGAATTCCCGATTTGCTTGGATGACCCGAAAGAGCGAGGAAACCATCACTCCGTGGAACTTCGAGGAAGTCGTGGACAAGGGTGCATCCGCGCAGAGCTTCATTGAGCGGATGACCAATTTTGATAAGAACCTTCCGAATGAAAAGGTCCTGCCAAAGCATTCGCTGCTCTACGAGTATTTCACCGTGTATAACGAACTGACTAAGGTCAAGTACGTGACGGAGGGAATGCGGAAGCCAGCCTTCCTCTCAGGGGAACAAAAGAAGGCTATCGTCGATTTGCTTTTTAAGACCAATCGTAAAGTGACTGTTAAGCAGCTGAAGGAGGATTATTTCAAGAAGATTGAATGTTTCGACTCCGTCGAGATCAGCGGCGTGGAAGATCGCTTTAACGCTTCCCTCGGTACCTACCACGACCTGCTCAAGATCATTAAGGACAAGGATTTCCTCGATAACGAGGAAAATGAGGACATCTTGGAAGATATTGTCCTCACGTTGACACTTTTTGAGGACCGCGAAATGATCGAGGAACGGCTCAAGACATATGCCCATTTGTTCGACGATAAGGTGATGAAGCAGCTGAAGCGGCGTCGATACACCGGATGGGGTCGCCTTAGCCGGAAGCTGATCAACGGCATTCGAGATAAGCAATCTGGTAAGACTATCTTGGATTTCCTTAAGTCGGACGGCTTCGCCAACCGCAATTTTATGCAGCTTATTCACGACGATTCCCTGACGTTCAAGGAGGACATCCAGAAGGCACAAGTCTCAGGACAAGGGGATTCCCTGCACGAGCATATCGCCAACCTGGCTGGATCCCCGGCGATCAAGAAGGGGATTCTTCAGACCGTCAAGGTTGTCGACGAGCTGGTCAAGGTGATGGGCCGTCATAAGCCAGAAAACATCGTGATTGAGATGGCCCGAGAAAATCAGACCACTCAAAAGGGTCAGAAGAACAGCCGCGAGCGGATGAAGCGGATCGAGGAAGGCATTAAGGAACTTGGTTCTCAGATCCTGAAGGAGCACCCTGTTGAAAACACACAGCTCCAAAATGAGAAGCTGTATCTCTACTATTTGCAAAATGGACGCGACATGTACGTCGATCAGGAGCTCGACATTAACCGGTTGTCGGACTACGATGTTGACCATATCGTCCCGCAATCCTTCCTTAAGGACGATAGCATTGATAACAAGGTGCTGACTCGCTCAGATAAGAACCGGGGCAAGTCCGACAATGTTCCAAGCGAGGAAGTGGTTAAGAAGATGAAGAACTACTGGCGCCAATTGCTTAATGCCAAGCTCATCACACAGCGCAAGTTTGACAACTTGACCAAGGCCGAGCGGGGAGGGCTGAGTGAACTCGATAAGGCTGGCTTCATCAAGCGTCAACTCGTGGAGACGCGACAGATCACAAAGCACGTTGCTCAGATTCTGGACTCCCGGATGAACACAAAGTACGACGAGAATGATAAGCTCATCCGTGAAGTTAAGGTCATTACCCTCAAGTCTAAGTTGGTGTCGGATTTCCGCAAGGACTTCCAATTTTATAAGGTTCGGGAGATCAACAATTATCACCATGCACATGATGCGTACCTCAACGCAGTCGTGGGAACTGCGCTCATCAAGAAGTATCCCAAGTTGGAGTCCGAATTCGTCTACGGGGATTATAAGGTTTACGACGTCCGCAAGATGATCGCCAAGAGTGAGCAGGAAATTGGCAAGGCCACGGCTAAGTATTTCTTTTACTCCAACATCATGAATTTCTTTAAGACGGAGATCACACTCGCCAATGGAGAAATCCGTAAGCGACCTTTGATTGAGACCAACGGCGAGACTGGTGAAATCGTTTGGGATAAGGGGCGCGACTTCGCTACCGTGCGGAAGGTTCTGAGCATGCCGCAAGTCAATATCGTCAAGAAAACCGAGGTGCAGACAGGCGGTTTCTCTAAGGAATCGATTCTTCCAAAGCGTAACTCTGACAAGCTGATCGCTCGAAAGAAGGATTGGGACCCCAAGAAGTATGGAGGGTTCGATTCTCCTACAGTGGCATACTCGGTTCTCGTTGTCGCGAAGGTTGAGAAGGGAAAGTCTAAGAAGCTGAAGTCGGTCAAGGAACTGCTCGGGATCACCATTATGGAGCGCTCCAGCTTCGAAAAGAATCCCATCGACTTTCTCGAGGCCAAGGGCTATAAGGAAGTCAAGAAGGATCTTATCATTAAGCTGCCTAAGTACTCTTTGTTCGAGCTTGAAAACGGTCGAAAGCGAATGCTCGCATCGGCAGGAGAGTTGCAGAAGGGGAATGAATTGGCACTTCCCTCAAAGTACGTGAACTTCCTGTATCTCGCGTCCCACTACGAGAAGCTGAAGGGTAGCCCTGAGGACAACGAACAGAAGCAACTTTTTGTTGAGCAACACAAGCATTATCTGGATGAGATCATTGAACAGATTTCAGAGTTCAGTAAGCGCGTCATCCTCGCCGATGCTAATCTCGACAAGGTGTTGTCGGCCTACAACAAGCACCGTGACAAGCCGATCCGAGAGCAGGCTGAAAATATCATTCATCTGTTCACCCTCACTAACTTGGGAGCACCAGCAGCGTTCAAGTATTTTGATACGACAATCGACCGTAAGCGATACACGTCCACAAAGGAGGTGCTTGATGCGACCCTGATTCATCAATCCATCACTGGGCTCTATGAAACCCGTATCGACCTTAGTCAACTGGGGGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
SV40 NLS — nuclear localization signal of SV40 (si… 13061..13082 Fwd
DNA Sequence
CCCAAGAAGAAGCGCAAGGTC
Protein Sequence
PKKKRKV
intron 1
Label Gene Product Position Strand
gpdA intron gpdA — 7483..7599 Fwd
DNA Sequence
GTAAGTACTTTGCTACATCCATACTCCATCCTTCCCATCCCTTATTCCTTTGAACCTTTCAGTTCGAGCTTTCCCACTTCATCGCAGCTTGACTAACAGCTACCCCGCTTGAGCAG
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 859..951 Rev
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAAT
protein_bind 2
Label Gene Product Position Strand
FRT (minimal) — — 1243..1277 Fwd
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
FRT (minimal) — — 15826..15860 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
rep_origin 2
Label Gene Product Position Strand
AMA1 — — 1589..6838 Fwd
DNA Sequence
AGCTTATTTTTTGTATACTGTTTTGTGATAGCACGAAGTTTTTCCACGGTATCTTGTTAAAAATATATATTTGTGGCGGGCTTACCTACATCAAATTAATAAGAGACTAATTATAAACTAAACACACAAGCAAGCTACTTTAGGGTAAAAGTTTATAAATGCTTTTGACGTATAAACGTTGCTTGTATTTATTATTACAATTAAAGGTGGATAGAAAACCTAGAGACTAGTTAGAAACTAATCTCAGGTTTGCGTTAAACTAAATCAGAGCCCGAGAGGTTAACAGAACCTAGAAGGGGACTAGATATCCGGGTAGGGAAACAAAAAAAAAAANCAAGACAGCCACATATTAGGGAGACTAGTTAGAAGCTAGTTCCAGGACTAGGAAAATAAAAGACAATGATACCACAGTCTAGTTGACAACTAGATAGATTCTAGATTGAGGCCAAAGTCTCTGAGATCCAGGTTAGTTGCAACTAATACTAGTTAGTATCTAGTCTCCTATAACTCTGAAGCTAGAATAACTTACTACTATTATCCTCACCACTGTTCAGCTGCGCAAACGGAGTGATTGCAAGGTGTTCAGAGACTAGTTATTGACTAGTCAGTGACTAGCAATAACTAACAAGGTATTAACCTACCATGTCTGCCATCACCCTGCACTTCCTCGGGCTCAGCAGCCTTTTCCTCCTCATTTTCATGCTCATTTTCCTTGTTTAAGACTGTGACTAGTCAAAGACTAGTCCAGAACCACAAAGGAGAAATGTCTTACCACTTTCTTCATTGCTTGTCTCTTTTGCATTATCCATGTCTGCAACTAGTTAGAGTCTAGTTAGTGACTAGTCCGACGAGGACTTGCTTGTCTCCGGATTGTTGGAGGAACTCTCCAGGGCCTCAAGATCCACAACAGAGCCTTCTAGAAGACTGGTCAATAACTAGTTGGTCTTTGTCTGAGTCTGACTTACGAGGTTGCATACTCGCTCCCTTTGCCTCGTCAATCGATGAGAAAAAGCGCCAAAACTCGCAATATGGCTTTGAACCACACGGTGCTGAGACTAGTTAGAATCTAGTCCCAAACTAGCTTGGATAGCTTACCTTTGCCCTTTGCGTTGCGACAGGTCTTGCAGGGTATGGTTCCTTTCTCACCAGCTGATTTAGCTGCCTTGCTACCCTCACGGCGGATCTGCATAAAGAGTGGCTAGAGGTTATAAATTAGCACTGATCCTAGGTACGGGGCTGAATGTAACTTGCCYTTCCTTTCTCATCGCGCGGCAAGACAGGCTTGCTCAAATTCCTACCAGTCACAGGGGTATGCACGGCGTACGGACCACTTGAACTAGTCACAGATTAGTTAGCAACTAGTCTGCATTGAATGGCTGTACTTACGGGCCCTCGCCATTGTCCTGATCATTTCCAGCTTCACCCTCGTTGCTGCAAAGTAGTTAGTGACTAGTCAAGGACTAGTTGAAATGGGAGAAGAAACTCACGAATTCTCGACACCCTTAGTATTGTGGTCCTTGGACTTGGTGCTGCTATATATTAGCTAATACACTAGTTAGACTCACAGAAACTTACGCAGCTCGCTTGCGCTTCTTGGTAGGAGTCGGGGTTGGGAGAACAGTGCCTTCAAACAAGCCTTCATACCATGCTACTTGACTAGTCAGGGACTAGTCACCAAGTAATCTAGATAGGACTTGCCTTTGGCCTCCATCAGTTCCTTCATAGTGGGAGGTCCATTGTGCAATGTAAACTCCATGCCGTGGGAGTTCTTGTCCTTCAAGTGCTTGACCAATATGTTTCTGTTGGCAGAGGGAACCTGTCAACTAGTTAATAACTAGTCAGAAACTAGTATAGCAGTAGACTCACTGTACGCTTGAGGCATCCCTTCACTCGGCAGTAGACTTCATATGGATGGATATCAGGCACGCCATTGTCGTCCTGTGGACTAGTCAGTAACTAGGCTTAAAGCTAGTCGGGTCGGCTTACTATCTTGAAATCCGGCAGCGTAAGCTCCCCGTCCTTAACTGCCTCGAGATAGTGACAGTACTCTGGGGACTTTCGGAGATCGTTATCGCGAATGCTCGGCATACTAATCGTTGACTAGTCTTGGACTAGTCCCGAGCAAAAAGGATTGGAGGAGGAGGAGGAAGGTGAGAGTGAGACAAAGAGCGAAATAAGAGCTTCAAAGGCTATCTCTAAGCAGTATGAAGGTTAAGTATCTAGTTCTTGACTAGATTTAAAAGAGATTTCGACTAGTTATGTACCTGGAGTTTGGATATAGGAATGTGTTGTGGTAACGAAATGTAAGGGAGAGGAAAGAAAAAGTCGGTCAAGAGGTAACTCTAAGTCGGCCATTCCTTTTTGGGAGGCGCTAACCATAAACGGCATGGTCGACTTAGAGTTAGCTCAGGGAATTTAGGGAGTTATCTGCGACCACCGAGGAACGGCGGAATGCCAAAGAATCCCGATGGAGCTCTAGCTGGCGGTTGACAACCCCACCTTTTGGCGTTTCTGCGGCGTTGCAGGCGGGACTGGATACTTCGTAGAACCAGAAAGGCAAGGCAGAACGCGCTCAGCAAGAGTGTTGGAAGTGATAGCATGATGTGCCTTGTTAACTAGGTCAAAATCTGCAGTATGCTTGATGTTATCCAAAGTGTGAGAGAGGAAGGTCCAAACATACACGATTGGGAGAGGGCCTAGGTATAAGAGTTTTTGAGTAGAACGCATGTGAGCCCAGCCATCTCGAGGAGATTAAACACGGGCCGGCATTTGATGGCTATGTTAGTACCCCAATGGAAACGGTGAGAGTCCAGTGGTCGCAGATAACTCCCTAAATTCCCTGAGCTAACTCTAAGTCGACCATGCCGTTTATGGTTAGCGCCTCCCAAAAAGGAATGGCCGACTTAGAGTTACCTCTTGACCGACTTTTTCTTTCCTCCCCCTTACATTTCGTTACCACAACACATTCCTATATCCAAACTCCAGGTACATAACTAGTCGAAATCTCTTTTAAATCTAGTCAAGAACTAGATACTTAACCTTCATACTGCTTAGAGATAGCCTTTGAAGCTCTTATTTCGCTCTTTGTCTCACTCTCACCTTCCTCCTCCTCCTCCAATCCTTTTTGCTCGGGACTAGTCCAAGACTAGTCAACGATTAGTATGCCGAGCATTCGCGATAACGATCTCCGAAAGTCCCCAGAGTACTGTCACTATCTCGAGGCAGTTAAGGACGGGGAGCTTACGCTGCCGGATTTCAAGATAGTAAGCCGACCCGACTAGCTTTAAGCCTAGTTACTGACTAGTCCACAGGACGACAATGGCGTGCCTGATATCCATCCATATGAAGTCTACTGCCGAGTGAAGGGATGCCTCAAGCGTACAGTGAGTCTACTGCTATACTAGTTTCTGACTAGTTATTAACTAGTTGACAGGTTCCCTCTGCCAACAGAAACATATTGGTCAAGCACTTGAAGGACAAGAACTCCCACGGCATGGAGTTTACATTGCACAATGGACCTCCCACTATGAAGGAACTGATGGAGGCCAAAGGCAAGTCCTATCTAGATTACTTGGTGACTAGTCCCTGACTAGTCAAGTAGCATGGTATGAAGGCTTGTTTGAAGGCACTGTTCTCCCAACCCCGACTCCTACCAAGAAGCGCAAGCGAGCTGCGTAAGTTTCTGTGAGTCTAACTAGTGTATTAGCTAATATATAGCAGCACCAAGTCCAAGGACCACAATACTAAGGGTGTCGAGAATTCGTGAGTTTCTTCTCCCATTTCAACTAGTCCTTGACTAGTCACTAACTACTTTGCAGCAACGAGGGTGAAGCTGGAAATGATCAGGACAATGGCGAGGGCCCGTAAGTACAGCCATTCAATGCAGACTAGTTGCTAACTAATCTGTGACTAGTTCAAGTGGTCCGTACGCCGTGCATACCCCTGTGACTGGTAGGAATTTGAGCAAGCCTGTCTTGCCGCGCGATGAGAAAGGAARGGCAAGTTACATTCAGCCCCGTACCTAGGATCAGTGCTAATTTATAACCTCTAGCCACTCTTTATGCAGATCCGCCGTGAGGGTAGCAAGGCAGCTAAATCAGCTGGTGAGAAAGGAACCATACCCTGCAAGACCTGTCGCAACGCAAAGGGCAAAGGTAAGCTATCCAAGCTAGTTTGGGACTAGATTCTAACTAGTCTCAGCACCGTGTGGTTCAAAGCCATATTGCGAGTTTTGGCGCTTTTTCTCATCGATTGACGAGGCAAAGGGAGCGAGTATGCAACCTCGTAAGTCAGACTCAGACAAAGACCAACTAGTTATTGACCAGTCTTCTAGAAGGCTCTGTTGTGGATCTTGAGGCCCTGGAGAGTTCCTCCAACAATCCGGAGACAAGCAAGTCCTCGTCGGACTAGTCACTAACTAGACTCTAACTAGTTGCAGACATGGATAATGCAAAAGAGACAAGCAATGAAGAAAGTGGTAAGACATTTCTCCTTTGTGGTTCTGGACTAGTCTTTGACTAGTCACAGTCTTAAACAAGGAAAATGAGCATGAAAATGAGGAGGAAAAGGCTGCTGAGCCCGAGGAAGTGCAGGGTGATGGCAGACATGGTAGGTTAATACCTTGTTAGTTATTGCTAGTCACTGACTAGTCAATAACTAGTCTCTGAACACCTTGCAATCACTCCGTTTGCGCAGCTGAACAGTGGTGAGGATAATAGTAGTAAGTTATTCTAGCTTCAGAGTTATAGGAGACTAGATACTAACTAGTATTAGTTGCAACTAACCTGGATCTCAGAGACTTTGGCCTCAATCTAGAATCTATCTAGTTGTCAACTAGACTGTGGTATCATTGTCTTTTATTTTCCTAGTCCTGGAACTAGCTTCTAACTAGTCTCCCTAATATGTGGCTGTCTTGTTTTTTTTTTTTGTTTCCCTACCCGGATATCTAGTCCCCTTCTAGGTTCTGTTAACCTCTCGGGCTCTGATTTAGTTTAACGCAAACCTGAGATTAGTTTCTAACTAGTCTCTAGGTTTTCTATCCACCTTTAATTGTAATAATAAATACAAGCAACGTTTATACGTCAAAAGCATTTATAAACTTTTACCCTAAAGTAGCTTGCTTGTGTGTTTAGTTTATAATTAGTCTCTTATTAATTTGATGTAGGTAAGCCCGCCACAAATATATATTTTTAACAAGATACCGTGGAAAAACTTCGTGCTATCACAAAACAGTATACAAAAAATAAGCT
ori — — 16615..17204 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA