pFC333

87844 Synthetic Bacterial Expression

Plasmid Information

Purpose
AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
Depositor
Uffe Mortensen
Article
Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
Tags
SV40 NLS
Promoter
Aspergillus nidulans tef1 promoter and Aspergillu…
Available Since
May 9, 2017
Availability
Available

Sequence Information

Length
15,668 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 4
Label Gene Product Position Strand
AmpR bla beta-lactamase 1561..2422 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGTAGCGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 9574..13678 Fwd
DNA Sequence
ATGGACAAGAAGTATAGCATCGGGCTGGACATTGGAACGAACTCGGTTGGTTGGGCTGTGATTACGGACGAATACAAGGTGCCATCCAAGAAGTTTAAGGTCCTGGGAAACACCGACCGTCACTCAATCAAGAAGAATCTCATTGGAGCCCTGCTCTTCGATAGTGGGGAGACCGCCGAAGCTACTCGACTGAAGCGAACGGCTCGCCGGCGTTATACACGACGCAAGAATCGCATCTGCTACCTCCAGGAGATTTTCAGCAACGAAATGGCTAAGGTTGATGACTCATTCTTTCATCGACTCGAAGAAAGTTTCTTGGTCGAGGAGGATAAGAAGCACGAGCGCCATCCGATCTTTGGTAACATTGTGGATGAGGTTGCCTATCACGAAAAGTACCCAACTATCTATCATCTTCGTAAGAAGCTGGTCGATAGCACGGACAAGGCTGATTTGCGACTTATCTACCTGGCACTCGCGCACATGATTAAGTTCCGCGGCCATTTTCTTATCGAGGGTGACCTGAACCCCGATAATTCTGACGTTGATAAGCTCTTCATCCAGTTGGTCCAAACCTACAATCAGCTGTTTGAGGAAAACCCTATTAATGCATCTGGCGTGGACGCCAAGGCTATCCTTTCGGCGCGCCTGTCTAAGTCGCGGCGTTTGGAGAACCTTATCGCACAACTCCCCGGCGAAAAGAAGAACGGCCTCTTCGGTAATTTGATTGCGTTGTCACTTGGTCTGACTCCTAACTTCAAGAGTAATTTTGACCTGGCAGAGGATGCGAAGCTCCAGTTGTCTAAGGATACGTATGATGACGATCTCGACAACTTGCTTGCCCAAATCGGTGACCAGTACGCTGATCTTTTCCTGGCCGCTAAGAATCTCTCAGATGCAATCCTGCTCAGTGACATTTTGCGGGTCAACACCGAGATTACTAAGGCCCCCCTGTCAGCTAGTATGATCAAGCGGTATGATGAGCACCATCAGGACCTCACCTTGCTTAAGGCCCTCGTGCGTCAGCAATTGCCTGAGAAGTATAAGGAAATCTTCTTTGACCAATCCAAGAACGGATACGCAGGGTATATTGATGGCGGTGCGAGCCAGGAGGAATTCTACAAGTTTATCAAGCCGATTTTGGAGAAGATGGACGGCACTGAGGAACTGCTCGTCAAGCTGAATCGCGAAGATTTGCTTCGTAAGCAACGAACGTTCGACAACGGCTCCATCCCGCACCAGATTCATCTGGGCGAGCTCCACGCCATCCTTCGACGCCAGGAAGATTTCTACCCATTTCTGAAGGACAACCGTGAGAAGATCGAAAAGATTCTTACATTCCGAATCCCCTACTATGTGGGACCTTTGGCCCGTGGGAATTCCCGATTTGCTTGGATGACCCGAAAGAGCGAGGAAACCATCACTCCGTGGAACTTCGAGGAAGTCGTGGACAAGGGTGCATCCGCGCAGAGCTTCATTGAGCGGATGACCAATTTTGATAAGAACCTTCCGAATGAAAAGGTCCTGCCAAAGCATTCGCTGCTCTACGAGTATTTCACCGTGTATAACGAACTGACTAAGGTCAAGTACGTGACGGAGGGAATGCGGAAGCCAGCCTTCCTCTCAGGGGAACAAAAGAAGGCTATCGTCGATTTGCTTTTTAAGACCAATCGTAAAGTGACTGTTAAGCAGCTGAAGGAGGATTATTTCAAGAAGATTGAATGTTTCGACTCCGTCGAGATCAGCGGCGTGGAAGATCGCTTTAACGCTTCCCTCGGTACCTACCACGACCTGCTCAAGATCATTAAGGACAAGGATTTCCTCGATAACGAGGAAAATGAGGACATCTTGGAAGATATTGTCCTCACGTTGACACTTTTTGAGGACCGCGAAATGATCGAGGAACGGCTCAAGACATATGCCCATTTGTTCGACGATAAGGTGATGAAGCAGCTGAAGCGGCGTCGATACACCGGATGGGGTCGCCTTAGCCGGAAGCTGATCAACGGCATTCGAGATAAGCAATCTGGTAAGACTATCTTGGATTTCCTTAAGTCGGACGGCTTCGCCAACCGCAATTTTATGCAGCTTATTCACGACGATTCCCTGACGTTCAAGGAGGACATCCAGAAGGCACAAGTCTCAGGACAAGGGGATTCCCTGCACGAGCATATCGCCAACCTGGCTGGATCCCCGGCGATCAAGAAGGGGATTCTTCAGACCGTCAAGGTTGTCGACGAGCTGGTCAAGGTGATGGGCCGTCATAAGCCAGAAAACATCGTGATTGAGATGGCCCGAGAAAATCAGACCACTCAAAAGGGTCAGAAGAACAGCCGCGAGCGGATGAAGCGGATCGAGGAAGGCATTAAGGAACTTGGTTCTCAGATCCTGAAGGAGCACCCTGTTGAAAACACACAGCTCCAAAATGAGAAGCTGTATCTCTACTATTTGCAAAATGGACGCGACATGTACGTCGATCAGGAGCTCGACATTAACCGGTTGTCGGACTACGATGTTGACCATATCGTCCCGCAATCCTTCCTTAAGGACGATAGCATTGATAACAAGGTGCTGACTCGCTCAGATAAGAACCGGGGCAAGTCCGACAATGTTCCAAGCGAGGAAGTGGTTAAGAAGATGAAGAACTACTGGCGCCAATTGCTTAATGCCAAGCTCATCACACAGCGCAAGTTTGACAACTTGACCAAGGCCGAGCGGGGAGGGCTGAGTGAACTCGATAAGGCTGGCTTCATCAAGCGTCAACTCGTGGAGACGCGACAGATCACAAAGCACGTTGCTCAGATTCTGGACTCCCGGATGAACACAAAGTACGACGAGAATGATAAGCTCATCCGTGAAGTTAAGGTCATTACCCTCAAGTCTAAGTTGGTGTCGGATTTCCGCAAGGACTTCCAATTTTATAAGGTTCGGGAGATCAACAATTATCACCATGCACATGATGCGTACCTCAACGCAGTCGTGGGAACTGCGCTCATCAAGAAGTATCCCAAGTTGGAGTCCGAATTCGTCTACGGGGATTATAAGGTTTACGACGTCCGCAAGATGATCGCCAAGAGTGAGCAGGAAATTGGCAAGGCCACGGCTAAGTATTTCTTTTACTCCAACATCATGAATTTCTTTAAGACGGAGATCACACTCGCCAATGGAGAAATCCGTAAGCGACCTTTGATTGAGACCAACGGCGAGACTGGTGAAATCGTTTGGGATAAGGGGCGCGACTTCGCTACCGTGCGGAAGGTTCTGAGCATGCCGCAAGTCAATATCGTCAAGAAAACCGAGGTGCAGACAGGCGGTTTCTCTAAGGAATCGATTCTTCCAAAGCGTAACTCTGACAAGCTGATCGCTCGAAAGAAGGATTGGGACCCCAAGAAGTATGGAGGGTTCGATTCTCCTACAGTGGCATACTCGGTTCTCGTTGTCGCGAAGGTTGAGAAGGGAAAGTCTAAGAAGCTGAAGTCGGTCAAGGAACTGCTCGGGATCACCATTATGGAGCGCTCCAGCTTCGAAAAGAATCCCATCGACTTTCTCGAGGCCAAGGGCTATAAGGAAGTCAAGAAGGATCTTATCATTAAGCTGCCTAAGTACTCTTTGTTCGAGCTTGAAAACGGTCGAAAGCGAATGCTCGCATCGGCAGGAGAGTTGCAGAAGGGGAATGAATTGGCACTTCCCTCAAAGTACGTGAACTTCCTGTATCTCGCGTCCCACTACGAGAAGCTGAAGGGTAGCCCTGAGGACAACGAACAGAAGCAACTTTTTGTTGAGCAACACAAGCATTATCTGGATGAGATCATTGAACAGATTTCAGAGTTCAGTAAGCGCGTCATCCTCGCCGATGCTAATCTCGACAAGGTGTTGTCGGCCTACAACAAGCACCGTGACAAGCCGATCCGAGAGCAGGCTGAAAATATCATTCATCTGTTCACCCTCACTAACTTGGGAGCACCAGCAGCGTTCAAGTATTTTGATACGACAATCGACCGTAAGCGATACACGTCCACAAAGGAGGTGCTTGATGCGACCCTGATTCATCAATCCATCACTGGGCTCTATGAAACCCGTATCGACCTTAGTCAACTGGGGGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
SV40 NLS — nuclear localization signal of SV40 (si… 13681..13702 Fwd
DNA Sequence
CCCAAGAAGAAGCGCAAGGTC
Protein Sequence
PKKKRKV
BleoR Sh ble from Streptoalloteichus hindustanus antibiotic-binding protein 14527..14902 Fwd
DNA Sequence
ATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGA
Protein Sequence
MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQD
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 2422..2514 Rev
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAAT
protein_bind 2
Label Gene Product Position Strand
FRT (minimal) — — 62..96 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
FRT (minimal) — — 2806..2840 Fwd
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
rep_origin 2
Label Gene Product Position Strand
ori — — 851..1440 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
AMA1 — — 3152..8403 Fwd
DNA Sequence
AGCTTATTTTTTGTATACTGTTTTGTGATAGCACGAAGTTTTTCCACGGTATCTTGTTAAAAATATATATTTGTGGCGGGCTTACCTACATCAAATTAATAAGAGACTAATTATAAACTAAACACACAAGCAAGCTACTTTAGGGTAAAAGTTTATAAATGCTTTTGACGTATAAACGTTGCTTGTATTTATTATTACAATTAAAGGTGGATAGAAAACCTAGAGACTAGTTAGAAACTAATCTCAGGTTTGCGTTAAACTAAATCAGAGCCCGAGAGGTTAACAGAACCTAGAAGGGGACTAGATATCCGGGTAGGGAAACAAAAAAAAAAAACAAGACAGCCACATATTAGGGAGACTAGTTAGAAGCTAGTTCCAGGACTAGGAAAATAAAAGACAATGATACCACAGTCTAGTTGACAACTAGATAGATTCTAGATTGAGGCCAAAGTCTCTGAGATCCAGGTTAGTTGCAACTAATACTAGTTAGTATCTAGTCTCCTATAACTCTGAAGCTAGAATAACTTACTACTATTATCCTCACCACTGTTCAGCTGCGCAAACGGAGTGATTGCAAGGTGTTCAGAGACTAGTTATTGACTAGTCAGTGACTAGCAATAACTAACAAGGTATTAACCTACCATGTCTGCCATCACCCTGCACTTCCTCGGGCTCAGCAGCCTTTTCCTCCTCATTTTCATGCTCATTTTCCTTGTTTAAGACTGTGACTAGTCAAAGACTAGTCCAGAACCACAAAGGAGAAATGTCTTACCACTTTCTTCATTGCTTGTCTCTTTTGCATTATCCATGTCTGCAACTAGTTAGAGTCTAGTTAGTGACTAGTCCGACGAGGACTTGCTTGTCTCCGGATTGTTGGAGGAACTCTCCAGGGCCTCAAGATCCACAACAGAGCCTTCTAGAAGACTGGTCAATAACTAGTTGGTCTTTGTCTGAGTCTGACTTACGAGGTTGCATACTCGCTCCCTTTGCCTCGTCAATCGATGAGAAAAAGCGCCAAAACTCGCAATATGGCTTTGAACCACACGGTGCTGAGACTAGTTAGAATCTAGTCCCAAACTAGCTTGGATAGCTTACCTTTGCCCTTTGCGTTGCGACAGGTCTTGCAGGGTATGGTTCCTTTCTCACCAGCTGATTTAGCTGCCTTGCTACCCTCACGGCGGATCTGCATAAAGAGTGGCTAGAGGTTATAAATTAGCACTGATCCTAGGTACGGGGCTGAATGTAACTTGCCYTTCCTTTCTCATCGCGCGGCAAGACAGGCTTGCTCAAATTCCTACCAGTCACAGGGGTATGCACGGCGTACGGACCACTTGAACTAGTCACAGATTAGTTAGCAACTAGTCTGCATTGAATGGCTGTACTTACGGGCCCTCGCCATTGTCCTGATCATTTCCAGCTTCACCCTCGTTGCTGCAAAGTAGTTAGTGACTAGTCAAGGACTAGTTGAAATGGGAGAAGAAACTCACGAATTCTCGACACCCTTAGTATTGTGGTCCTTGGACTTGGTGCTGCTATATATTAGCTAATACACTAGTTAGACTCACAGAAACTTACGCAGCTCGCTTGCGCTTCTTGGTAGGAGTCGGGGTTGGGAGAACAGTGCCTTCAAACAAGCCTTCATACCATGCTACTTGACTAGTCAGGGACTAGTCACCAAGTAATCTAGATAGGACTTGCCTTTGGCCTCCATCAGTTCCTTCATAGTGGGAGGTCCATTGTGCAATGTAAACTCCATGCCGTGGGAGTTCTTGTCCTTCAAGTGCTTGACCAATATGTTTCTGTTGGCAGAGGGAACCTGTCAACTAGTTAATAACTAGTCAGAAACTAGTATAGCAGTAGACTCACTGTACGCTTGAGGCATCCCTTCACTCGGCAGTAGACTTCATATGGATGGATATCAGGCACGCCATTGTCGTCCTGTGGACTAGTCAGTAACTAGGCTTAAAGCTAGTCGGGTCGGCTTACTATCTTGAAATCCGGCAGCGTAAGCTCCCCGTCCTTAACTGCCTCGAGATAGTGACAGTACTCTGGGGACTTTCGGAGATCGTTATCGCGAATGCTCGGCATACTAATCGTTGACTAGTCTTGGACTAGTCCCGAGCAAAAAGGATTGGAGGAGGAGGAGGAAGGTGAGAGTGAGACAAAGAGCGAAATAAGAGCTTCAAAGGCTATCTCTAAGCAGTATGAAGGTTAAGTATCTAGTTCTTGACTAGATTTAAAAGAGATTTCGACTAGTTATGTACCTGGAGTTTGGATATAGGAATGTGTTGTGGTAACGAAATGTAAGGGGGAGGAAAGAAAAAGTCGGTCAAGAGGTAACTCTAAGTCGGCCATTCCTTTTTGGGAGGCGCTAACCATAAACGGCATGGTCGACTTAGAGTTAGCTCAGGGAATTTAGGGAGTTATCTGCGACCACCGAGGAACGGCGGAATGCCAAAGAATCCCGATGGAGCTCTAGCTGGCGGTTGACAACCCCACCTTTTGGCGTTTCTGCGGCGTTGCAGGCGGGACTGGATACTTCGTAGAACCAGAAAGGCAAGGCAGAACGCGCTCAGCAAGAGTGTTGGAAGTGATAGCATGATGTGCCTTGTTAACTAGGTCAAAATCTGCAGTATGCTTGATGTTATCCGAAGTGTGAGAGAGGAAGGTCCAAACATACACGATTGGGAGAGGGCCTAGGTATAAGAGTTTTTGAGTAGAACGCATGTGAGCCCAGCCATCTCGAGGAGATTAAACACGGGCCGGCATTTGATGGCTATGTTAGTACCCCAATGGAAACGGTGAGAGTCCAGTGGTCGCAGATAACTCCCTAAATTCCCTGAGCTAACTCTAAGTCGACCATGCCGTTTATGGTTAGCGCCTCCCAAAAAGGAATGGCCGACTTAGAGTTACCTCTTGACCGACTTTTTCTTTCCTCCCCCTTACATTTCGTTACCACAACACATTCCTATATCCAAACTCCAGGTACATAACTAGTCGAAATCTCTTTTAAATCTAGTCAAGAACTAGATACTTAACCTTCATACTGCTTAGAGATAGCCTTTGAAGCTCTTATTTCGCTCTTTGTCTCACTCTCACCTTCCTCCTCCTCCTCCAATCCTTTTTGCTCGGGACTAGTCCAAGACTAGTCAACGATTAGTATGCCGAGCATTCGCGATAACGATCTCCGAAAGTCCCCAGAGTACTGTCACTATCTCGAGGCAGTTAAGGACGGGGAGCTTACGCTGCCGGATTTCAAGATAGTAAGCCGACCCGACTAGCTTTAAGCCTAGTTACTGACTAGTCCACAGGACGACAATGGCGTGCCTGATATCCATCCATATGAAGTCTACTGCCGAGTGAAGGGATGCCTCAAGCGTACAGTGAGTCTACTGCTATACTAGTTTCTGACTAGTTATTAACTAGTTGACAGGTTCCCTCTGCCAACAGAAACATATTGGTCAAGCACTTGAAGGACAAGAACTCCCACGGCATGGAGTTTACATTGCACAATGGACCTCCCACTATGAAGGAACTGATGGAGGCCAAAGGCAAGTCCTATCTAGATTACTTGGTGACTAGTCCCTGACTAGTCAAGTAGCATGGTATGAAGGCTTGTTTGAAGGCACTGTTCTCCCAACCCCGACTCCTACCAAGAAGCGCAAGCGAGCTGCGTAAGTTTCTGTGAGTCTAACTAGTGTATTAGCTAATATATAGCAGCACCAAGTCCAAGGACCACAATACTAAGGGTGTCGAGAATTCGTGAGTTTCTTCTCCCATTTCAACTAGTCCTTGACTAGTCACTAACTACTTTGCAGCAACGAGGGTGAAGCTGGAAATGATCAGGACAATGGCGAGGGCCCGTAAGTACAGCCATTCAATGCAGACTAGTTGCTAACTAATCTGTGACTAGTTCAAGTGGTCCGTACGCCGTGCATACCCCTGTGACTGGTAGGAATTTGAGCAAGCCTGTCTTGCCGCGCGATGAGAAAGGAARGGCAAGTTACATTCAGCCCCGTACCTAGGATCAGTGCTAATTTATAACCTCTAGCCACTCTTTATGCAGATCCGCCGTGAGGGTAGCAAGGCAGCTAAATCAGCTGGTGAGAAAGGAACCATACCCTGCAAGACCTGTCGCAACGCAAAGGGCAAAGGTAAGCTATCCAAGCTAGTTTGGGACTAGATTCTAACTAGTCTCAGCACCGTGTGGTTCAAAGCCATATTGCGAGTTTTGGCGCTTTTTCTCATCGATTGACGAGGCAAAGGGAGCGAGTATGCAACCTCGTAAGTCAGACTCAGACAAAGACCAACTAGTTATTGACCAGTCTTCTAGAAGGCTCTGTTGTGGATCTTGAGGCCCTGGAGAGTTCCTCCAACAATCCGGAGACAAGCAAGTCCTCGTCGGACTAGTCACTAACTAGACTCTAACTAGTTGCAGACATGGATAATGCAAAAGAGACAAGCAATGAAGAAAGTGGTAAGACATTTCTCCTTTGTGGTTCTGGACTAGTCTTTGACTAGTCACAGTCTTAAACAAGGAAAATGAGCATGAAAATGAGGAGGAAAAGGCTGCTGAGCCCGAGGAAGTGCAGGGTGATGGCAGACATGGTAGGTTAATACCTTGTTAGTTATTGCTAGTCACTGACTAGTCAATAACTAGTCTCTGAACACCTTGCAATCACTCCGTTTGCGCAGCTGAACAGTGGTGAGGATAATAGTAGTAAGTTATTCTAGCTTCAGAGTTATAGGAGACTAGATACTAACTAGTATTAGTTGCAACTAACCTGGATCTCAGAGACTTTGGCCTCAATCTAGAATCTATCTAGTTGTCAACTAGACTGTGGTATCATTGTCTTTTATTTTCCTAGTCCTGGAACTAGCTTCTAACTAGTCTCCCTAATATGTGGCTGTCTTGTTTTTTTTTTTTTTGTTTCCCTACCCGGATATCTAGTCCCCTTCTAGGTTCTGTTAACCTCTCGGGCTCTGATTTAGTTTAACGCAAACCTGAGATTAGTTTCTAACTAGTCTCTAGGTTTTCTATCCACCTTTAATTGTAATAATAAATACAAGCAACGTTTATACGTCAAAAGCATTTATAAACTTTTACCCTAAAGTAGCTTGCTTGTGTGTTTAGTTTATAATTAGTCTCTTATTAATTTGATGTAGGTAAGCCCGCCACAAATATATATTTTTAACAAGATACCGTGGAAAAACTTCGTGCTATCACAAAACAGTATACAAAAAATAAGCT