pF714-pET28a-Hs-FL-METTL20-NHis wt

85115 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag
Depositor
Pal Falnes
Article
Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d
Insert
human full-length METTL20 (METTL20 Human)
Tags
6xHis
Promoter
T7
Available Since
Nov. 12, 2018
Availability
Available

Sequence Information

Length
919 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 52..70 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 79..97 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS