p1G108CP20X

85096 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses PCNA1 variant (G108C) fused to putidaredoxin in E. coli
Depositor
Teruyuki Nagamune
Article
Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
Insert
The G108C variant of PCNA1 fused to putidaredoxin
Tags
His6 tag
Mutation
G108C in PCNA1
Promoter
T7 promoter
Available Since
Nov. 29, 2016
Availability
Available

Sequence Information

Length
853 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 59..77 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 86..104 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS