pGS-3R112C/T180C

85095 Synthetic Bacterial Expression

Plasmid Information

Purpose
Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)
Depositor
Teruyuki Nagamune
Article
Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
Insert
DNA polymerase sliding clamp 3
Tags
His6 tag
Mutation
changed Arginine 112 to Cysteine and Threonine 18…
Promoter
T7 promoter
Available Since
Nov. 29, 2016
Availability
Available

Sequence Information

Length
1,019 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 59..77 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 86..104 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 945..963 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH