p3R112C/T180CHFm

85091 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli
Depositor
Teruyuki Nagamune
Article
Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
Insert
The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
Tags
His6 tag
Mutation
R112C/T180C in PCNA3, A74G in heme-FMN domain of …
Promoter
T7 promoter
Available Since
Dec. 12, 2016
Availability
Available

Sequence Information

Length
1,026 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 60..78 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 87..105 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS