p1G108CFa

85089 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli
Depositor
Teruyuki Nagamune
Article
Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
Insert
The G108C variant of PCNA1 fused to an FAD domain of P450 BM3
Tags
His6 tag
Mutation
G108C in PCNA1, A74G/C773S/C810S in FAD domain of…
Promoter
T7 promoter
Available Since
Nov. 29, 2016
Availability
Available

Sequence Information

Length
921 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 59..77 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 86..104 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS