pP450cam

85085 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses P450cam (CYP101A1) in E. coli
Depositor
Teruyuki Nagamune
Article
Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
Insert
P450cam
Tags
His6 tag
Promoter
T7 promoter
Available Since
Nov. 28, 2016
Availability
Available

Sequence Information

Length
895 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 60..78 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 87..105 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS