pET-UbcH10-DN

8502 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Joan Ruderman
Article
Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.
Insert
UbcH10 (UBE2C Human)
Tags
His
Mutation
Dominant Negative, C114S
Available Since
June 1, 2005
Availability
Available

Sequence Information

Length
899 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 588..606 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 672..720 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG