pAG416-Gal-GA50

84903 Synthetic Yeast Expression

Plasmid Information

Purpose
induce expression of dipeptide repeat GA in yeast
Depositor
Aaron Gitler
Article
Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi
Insert
GA50 (C9orf72 Synthetic)
Tags
FLAG and Myc
Promoter
GAL1
Available Since
Nov. 17, 2016
Availability
Available

Sequence Information

Length
731 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
HQ tag — HQHQHQ metal affinity tag 712..730 Rev
DNA Sequence
CACCAGCACCAGCACCAG
Protein Sequence
HQHQHQ
protein_bind 1
Label Gene Product Position Strand
attB1 mutant version of attB — 471..496 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT