pAG416-Gal-PA50 — PlasmidDB

Plasmid Information

Purpose

induce expression of dipeptide repeat PA in yeast

Depositor

Aaron Gitler

Article

Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

Insert

PA50 (C9orf72 Synthetic)

Length

630 bp

Topology

linear

Molecule Type

genomic DNA

CDS 2

Label	Gene	Product	Position	Strand
FLAG	—	FLAG(R) epitope tag, followed by an ent…	325..349	Rev
DNA Sequence GATTACAAGGATGACGACGATAAG Protein Sequence DYKDDDDK
Myc	—	Myc (human c-Myc proto-oncogene) epitop…	367..397	Rev
DNA Sequence GAGCAGAAACTCATCTCAGAAGAGGATCTG Protein Sequence EQKLISEEDL

protein_bind 1

Label	Gene	Product	Position	Strand
attB2	mutant version of attB	—	290..315	Fwd
DNA Sequence ACCACTTTGTACAAGAAAGCTGGGT

terminator 1

Label	Gene	Product	Position	Strand
CYC1 terminator	S. cerevisiae CYC1	—	3..193	Rev
DNA Sequence ATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAG