pCFJ1955 — PlasmidDB

Plasmid Information

Purpose

Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

C. elegans codon optimized tdTomato

Promoter

Peft-3

Available Since

Dec. 6, 2016

Availability

Available

Sequence Information

Length

1,034 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

promoter 1

Label	Gene	Product	Position	Strand
eft-3 promoter	eft-3	—	267..867	Fwd
DNA Sequence GCACCTTTGGTCTTTTATTGTCAACTTCCATTGGTTCTTCCATTGTTTCTGTTAAATTAATGAATTTTTCATAAAATAAAGACATTATACAATATAAAAATGAAGAATTTATTGAAAATAAACTGCCAGAGAGAAAAAGTATGCAACACTCCCGCCGAGAGTGTTTGAAATGGTGTACGGTACATTTTCGTGCTAGGAGTTAGATGTGCAGGCAGCAACGAGAGGGGGAGAGATTTTTTTGGGCCTTGTGAAATTAACGTGAGTTTTCTGGTCATCTGACTAATCATGTTGGTTTTTTGTTGGTTTATTTTGTTTTTATCTTTGTTTTTATCCAGATTAGGAAATTTAAATTTTATGAATTTATAATGAGGTCAAACATTCAGTCCCAGCGTTTTTCCTGTTCTCACTGTTTAGTCGAATTTTTATTTTAGGCTTTCAACAAATGTTCTAACTGTCTTATTTGTGACCTCACTTTTTATATTTTTTTAATTTTTAAAAATATTAGAAGTTTCTAGGATAATTTTTTCGACTTTTATTCTCTCTACCGTCCGCACTCTTCTTACTTTTAAATTAAATTGTTTTTTTTTCAGTTGGGAAACA

protein_bind 2

Label	Gene	Product	Position	Strand
attB4	mutant version of attB	—	246..267	Fwd
DNA Sequence CAACTTTGTATAGAAAAGTTG
attB1	mutant version of attB	—	892..917	Fwd
DNA Sequence GCAAGTTTGTACAAAAAAGCAGGCT