pCFJ1415 — PlasmidDB

Plasmid Information

Purpose

Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

C. elegans codon optimized GFP

Promoter

Peft-3

Available Since

Dec. 6, 2016

Availability

Available

Sequence Information

Length

999 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 1

Label	Gene	Product	Position	Strand
SV40 NLS	—	nuclear localization signal of SV40 (si…	907..928	Rev
DNA Sequence CCAAAGAAGAAGCGTAAGGTG Protein Sequence PKKKRKV

protein_bind 1

Label	Gene	Product	Position	Strand
attB1	mutant version of attB	—	930..955	Rev
DNA Sequence GCAAGTTTGTACAAAAAAGCAGGCT