pCFJ1137

84827 Caenorhabditis elegans Bacterial Expression

Plasmid Information

Purpose
Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
Depositor
Andrew Fire
Article
Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
Insert
C. elegans codon optimized GFP
Promoter
Peft-3
Available Since
Dec. 6, 2016
Availability
Available

Sequence Information

Length
1,048 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
SV40 NLS — nuclear localization signal of SV40 (si… 152..173 Rev
DNA Sequence
CCAAAGAAGAAGCGTAAGGTG
Protein Sequence
PKKKRKV
promoter 1
Label Gene Product Position Strand
eft-3 promoter eft-3 — 225..825 Rev
DNA Sequence
GCACCTTTGGTCTTTTATTGTCAACTTCCATTGGTTCTTCCATTGTTTCTGTTAAATTAATGAATTTTTCATAAAATAAAGACATTATACAATATAAAAATGAAGAATTTATTGAAAATAAACTGCCAGAGAGAAAAAGTATGCAACACTCCCGCCGAGAGTGTTTGAAATGGTGTACGGTACATTTTCGTGCTAGGAGTTAGATGTGCAGGCAGCAACGAGAGGGGGAGAGATTTTTTTGGGCCTTGTGAAATTAACGTGAGTTTTCTGGTCATCTGACTAATCATGTTGGTTTTTTGTTGGTTTATTTTGTTTTTATCTTTGTTTTTATCCAGATTAGGAAATTTAAATTTTATGAATTTATAATGAGGTCAAACATTCAGTCCCAGCGTTTTTCCTGTTCTCACTGTTTAGTCGAATTTTTATTTTAGGCTTTCAACAAATGTTCTAACTGTCTTATTTGTGACCTCACTTTTTATATTTTTTTAATTTTTAAAAATATTAGAAGTTTCTAGGATAATTTTTTCGACTTTTATTCTCTCTACCGTCCGCACTCTTCTTACTTTTAAATTAAATTGTTTTTTTTTCAGTTGGGAAACA
protein_bind 2
Label Gene Product Position Strand
attB1 mutant version of attB — 175..200 Rev
DNA Sequence
GCAAGTTTGTACAAAAAAGCAGGCT
attB4 mutant version of attB — 825..846 Rev
DNA Sequence
CAACTTTGTATAGAAAAGTTG