pET - FnR062.Y78N

84795 Homo sapiens Bacterial Expression

Plasmid Information

Purpose

bacterial expression of FnR.0.6.2-Y78N protein

Depositor

Benjamin Hackel

Article

Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374

Insert

FnR.0.6.2-Y78N

Tags

6xHis

Mutation

Y78N

Available Since

Jan. 29, 2021

Availability

Available

Sequence Information

Length

1,004 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	337..355	Fwd
DNA Sequence CACCATCACCATCATCAC Protein Sequence HHHHHH

rep_origin 1

Label	Gene	Product	Position	Strand
f1 ori	—	—	517..973	Fwd
DNA Sequence ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	433..481	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG