pET - FnR062.Y78D

84786 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of FnR.0.6.2-Y78D protein
Depositor
Benjamin Hackel
Article
Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374
Insert
FnR.0.6.2-Y78D
Tags
6xHis
Mutation
Y78D
Available Since
Jan. 29, 2021
Availability
Available

Sequence Information

Length
949 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 37..55 Rev
DNA Sequence
CACCATCACCATCATCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
T7 promoter — — 430..449 Rev
DNA Sequence
TAATACGACTCACTATAGG
lacI promoter lacI — 757..835 Fwd
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
protein_bind 1
Label Gene Product Position Strand
lac operator — — 405..430 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC