pET - FnR062.Y78

84783 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of FnR.0.6.2-Y78 protein
Depositor
Benjamin Hackel
Article
Johnson et al J. Chem. Educ. 2017, 94, 3, 367–374
Insert
FnR.0.6.2-Y78
Tags
6xHis
Available Since
Oct. 24, 2016
Availability
Available

Sequence Information

Length
909 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 41..59 Rev
DNA Sequence
CACCATCACCATCATCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
T7 promoter — — 434..453 Rev
DNA Sequence
TAATACGACTCACTATAGG
lacI promoter lacI — 761..839 Fwd
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
protein_bind 1
Label Gene Product Position Strand
lac operator — — 409..434 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC