pDEW8

84238 Synthetic Yeast Expression

Plasmid Information

Purpose
Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae
Depositor
David Weinberg
Article
DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1
Insert
HA-mRuby-P2A-eGFP
Tags
HA-mRuby and eGFP
Promoter
GPD
Available Since
Nov. 7, 2016
Availability
Available

Sequence Information

Length
1,022 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
HA HA (human influenza hemagglutinin) epit… 91..118 Fwd
DNA Sequence
TACCCATACGATGTTCCTGACTATGCG
Protein Sequence
YPYDVPDYA
mRuby2 monomeric red fluorescent protein deriv… 127..835 Fwd
DNA Sequence
GTGTCCAAAGGAGAGGAGTTAATCAAGGAAAACATGAGAATGAAAGTTGTCATGGAGGGCTCCGTTAATGGTCACCAATTCAAGTGTACAGGGGAAGGTGAAGGTAATCCTTACATGGGTACACAAACTATGAGAATTAAAGTAATTGAAGGCGGACCACTACCATTTGCATTTGACATTCTGGCAACGTCATTCATGTACGGATCACGAACTTTCATCAAGTACCCTAAAGGTATACCAGACTTTTTCAAGCAATCTTTTCCAGAGGGTTTTACATGGGAAAGGGTTACAAGATACGAAGATGGGGGTGTCGTCACAGTTATGCAAGATACTTCATTAGAAGATGGCTGCCTTGTCTATCATGTGCAAGTAAGAGGGGTGAATTTTCCTTCTAACGGACCTGTGATGCAGAAAAAGACCAAAGGTTGGGAACCAAATACTGAAATGATGTACCCAGCTGATGGAGGTTTGAGAGGCTACACACACATGGCGCTTAAAGTTGATGGTGGAGGTCATTTGTCTTGTAGTTTTGTTACCACTTATCGTTCTAAAAAGACTGTTGGCAATATCAAAATGCCAGGAATACATGCTGTAGACCACAGACTAGAAAGACTCGAAGAGAGCGATAACGAAATGTTCGTTGTACAGAGAGAGCATGCCGTAGCCAAATTTGCTGGCTTAGGCGGTGGTATGGATGAATTGTATAAG
Protein Sequence
VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFAGLGGGMDELYK
P2A 2A peptide from porcine teschovirus-1 p… 877..934 Fwd
DNA Sequence
GCAACAAACTTCTCCCTCTTGAAACAAGCAGGAGATGTTGAGGAAAATCCCGGGCCT
Protein Sequence
ATNFSLLKQAGDVEENPGP