pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

83947 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
gRNA cassette-carrying vector with natMX marker
Depositor
Irina Borodina
Article
Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d
Insert
gRNA targeting ADE2 gene
Available Since
Oct. 27, 2016
Availability
Available

Sequence Information

Length
1,030 bp
Topology
linear
Molecule Type
genomic DNA

Features

promoter 3
Label Gene Product Position Strand
SNR52 promoter 392..661 Rev
DNA Sequence
TCTTTGAAAAGATAATGTATGATTATGCTTTCACTCATATTTATACAGAAACTTGATGTTTTCTTTCGAGTATATACAAGGTGATTACATGTACGTTTGAAGTACAACTCTAGATTTTGTAGTGCCCTCTTGGGCTAGCGGTAAAGGTGCGCATTTTTTCACACCCTACAATGTTCTGTTCAAAAGATTTTGGTCAAACGCTGTAGAAGTGAAAGTTGGTGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATC
T3 promoter 678..697 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter 765..796 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
lac operator 741..758 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 810..832 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
terminator 2
Label Gene Product Position Strand
CYC1 terminator S. cerevisiae CYC1 20..268 Rev
DNA Sequence
TCATGTAATTAGTTATGTCACGCTTACGTTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGC
SUP4 terminator 273..293 Rev
DNA Sequence
TTTTTTTGTTTTTTATGTCT