pRSF-Ivy-his-hLYZ

83920 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.
Depositor
Karl Griswold
Article
Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do
Tags
Ivy protein has C-terminal hisx6 tag
Mutation
Ivy leader sequence deleted and replaced.
Promoter
T7 and t7
Available Since
Nov. 28, 2016
Availability
Available

Sequence Information

Length
836 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 22..40 Rev
DNA Sequence
CATCACCATCATCACCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
T7 promoter — — 501..520 Rev
DNA Sequence
TAATACGACTCACTATAGG
lacI promoter lacI — 565..643 Fwd
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
protein_bind 1
Label Gene Product Position Strand
lac operator — — 476..501 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC