pBiex PKC - Peptide 14 mCit mCer

83565 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
Depositor
Sivaraj Sivaramakrishnan
Article
Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
Insert
PKC alpha (PRKCA Human)
Tags
10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…
Mutation
Only catalytic domain of PKC (aa 335-672)
Promoter
T7 Promoter
Available Since
Nov. 3, 2016
Availability
Available

Sequence Information

Length
947 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 2
Label Gene Product Position Strand
T7 promoter — — 300..319 Rev
DNA Sequence
TAATACGACTCACTATAGG
IE1 promoter — — 332..924 Rev
DNA Sequence
CGATGTCTTTGTGATGCGCGCGACATTTTTGTAGGTTATTGATAAAATGAACGGATACGTTGCCCGACATTATCATTAAATCCTTGGCGTAGAATTTGTCGGGTCCATTGTCCGTGTGCGCTAGCATGCCCGTAACGGACCTCGTACTTTTGGCTTCAAAGGTTTTGCGCACAGACAAAATGTGCCACACTTGCAGCTCTGCATGTGTGCGCGTTACCACAAATCCCAACGGCGCAGTGTACTTGTTGTATGCAAATAAATCTCGATAAAGGCGCGGCGCGCGAATGCAGCTGATCACGTACGCTCCTCGTGTTCCGTTCAAGGACGGTGTTATCGACCTCAGATTAATGTTTATCGGCCGACTGTTTTCGTATCCGCTCACCAAACGCGTTTTTGCATTAACATTGTATGTCGGCGGATGTTCTATATCTAATTTGAATAAATAAACGATAACCGCGTTGGTTTTAGAGGGCATAATAAAAGAAATATTGTTATCGTGTTCGCCATTAGGGCAGTATAAATTGACGTTCATGTTGGATATTGTTTCAGTTGCAAGTTGACACTGGCGGCGACAAGATCGTGAACAACCAAG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 275..300 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC