Purpose
Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
Depositor
Sivaraj Sivaramakrishnan
Article
Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
Insert
PKC alpha (PRKCA Human)
Tags
10 nm ER/K Helix, FLAG purification tag, TEV Prot…
Mutation
Only catalytic domain of PKC (aa 335-672)
Promoter
T7 Promoter
Available Since
Nov. 16, 2016
Availability
Available
No features annotated for this plasmid.