pGEN25-Ub Hs

83232 Homo sapiens Bacterial Expression

Plasmid Information

Purpose

pHis-par2-Ub Hs

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

Ubiqutin (UBC Human)

Tags

His

Available Since

Nov. 22, 2016

Availability

Available

Sequence Information

Length

1,003 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

CDS 4

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	30..48	Rev
DNA Sequence CACCACCACCACCACCAC Protein Sequence HHHHHH
ubiquitin	Homo sapiens UBC	human ubiquitin	122..350	Rev
DNA Sequence ATGCAGATCTTCGTGAAGACTCTGACTGGTAAGACCATCACCCTCGAGGTGGAGCCCAGTGACACCATCGAGAATGTCAAGGCAAAGATCCAAGATAAGGAAGGCATTCCTCCTGATCAGCAGAGGTTGATCTTTGCCGGAAAACAGCTGGAAGATGGTCGTACCCTGTCTGACTACAACATCCAGAAAGAGTCCACCTTGCACCTGGTACTCCGTCTCAGAGGTGGG Protein Sequence MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
TEV site	—	tobacco etch virus (TEV) protease recog…	362..383	Rev
DNA Sequence GAAAACCTGTATTTTCAGGGC Protein Sequence ENLYFQG
6xHis	—	6xHis affinity tag	404..422	Rev
DNA Sequence CATCACCATCACCATCAC Protein Sequence HHHHHH

promoter 2

Label	Gene	Product	Position	Strand
T7 promoter	—	—	503..522	Rev
DNA Sequence TAATACGACTCACTATAGG
lacI promoter	lacI	—	830..908	Fwd
DNA Sequence GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT

protein_bind 1

Label	Gene	Product	Position	Strand
lac operator	—	—	478..503	Fwd
DNA Sequence GGAATTGTTATCCGCTCACAATTCC