pCDFDuet-MKK6-EE

82718 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.
Depositor
Kevin Janes
Article
Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl
Insert
MKK6 (MAP2K6 Human)
Mutation
Ser207 to Glu, Thr211 to Glu
Available Since
Feb. 28, 2019
Availability
Available

Sequence Information

Length
694 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 195..213 Fwd
DNA Sequence
CATCACCATCATCACCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 96..115 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 115..140 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC