pGEX-6P-1-GST-CD-ATLm(Cbx7)

82528 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.
Depositor
Xiaojun Ren
Insert
Chromobox Homolog 7 (CBX7 Human)
Tags
GST
Mutation
amino acids 1-84 with mutation of RKR70-72, K75, …
Available Since
Dec. 1, 2016
Availability
Available

Sequence Information

Length
995 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
HRV 3C site — recognition and cleavage site for human… 9..33 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 625..730 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT