pCS2-MT-mitfa

81231 Danio rerio Bacterial Expression

Plasmid Information

Purpose
expression of zebrafish mitfa
Depositor
James Lister, David Raible
Article
Lister et al Development. 1999 Sep;126(17):3757-67.
Insert
mitfa (mitfa Zebrafish)
Tags
6 myc tags
Mutation
contains amino acids 5-412
Promoter
sCMV IE94 promoter
Available Since
Aug. 15, 2016
Availability
Available

Sequence Information

Length
1,025 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 6
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 130..160 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 169..199 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 208..238 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 247..277 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 286..316 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 349..379 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
promoter 1
Label Gene Product Position Strand
SP6 promoter — — 62..81 Fwd
DNA Sequence
ATTTAGGTGACACTATAGA