pSALECTNK-TEM1(S70A,D179G)/csTEM1

81163 Synthetic Bacterial Expression

Plasmid Information

Purpose
pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1
Depositor
Timothy Whitehead
Article
Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p
Insert
TEM-1 beta-lactamase
Tags
Codon swapped TEM-1 from H26 to W290 and His6x
Mutation
Deleted the sec transport tag. Plasmid encodes H2…
Promoter
lac promoter
Available Since
Feb. 21, 2017
Availability
Available

Sequence Information

Length
861 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 152..170 Rev
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH