pET28-mTDGa.1

81051 Mus musculus Bacterial Expression

Plasmid Information

Purpose
bacterial expression of catalytically dead murine TDG
Depositor
Primo Schaer
Article
Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.
Insert
TDG (Tdg Mouse)
Tags
6HIS
Mutation
asparagine 151 changed to alanine
Promoter
T7
Available Since
Aug. 19, 2016
Availability
Available

Sequence Information

Length
798 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 46..64 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 73..91 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS