pTriEx-mVenus-Zdk1

81047 Synthetic Bacterial Expression

Plasmid Information

Purpose
mammalian expression of Zdk1
Depositor
Klaus Hahn
Article
Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n
Insert
Zdk1
Tags
6x His tag, FLAG, and mVenus
Available Since
Aug. 16, 2016
Availability
Available

Sequence Information

Length
994 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 7
Label Gene Product Position Strand
8xHis 8xHis affinity tag 199..223 Rev
DNA Sequence
CACCACCATCACCATCACCATCAC
Protein Sequence
HHHHHHHH
HSV tag HSV (herpes simplex virus) epitope tag 229..262 Rev
DNA Sequence
CAGCCAGAACTCGCCCCGGAAGACCCCGAGGAT
Protein Sequence
QPELAPEDPED
FLAG FLAG(R) epitope tag, followed by an ent… 301..325 Rev
DNA Sequence
GACTACAAGGACGACGACGACAAA
Protein Sequence
DYKDDDDK
TEV site tobacco etch virus (TEV) protease recog… 331..352 Rev
DNA Sequence
GAAAATCTGTATTTCCAGGGC
Protein Sequence
ENLYFQG
6xHis 6xHis affinity tag 358..376 Rev
DNA Sequence
CATCATCATCATCATCAT
Protein Sequence
HHHHHH
Zdk1 "Zdark" protein, derived from the Z sub… 394..571 Rev
DNA Sequence
ATGGTGGATAACAAATTCAATAAAGAAAAGACGCGTGCCGGTGCGGAAATCCATTCTCTGCCAAACCTGAATGTTGAGCAGAAGTTTGCCTTCATCGTGAGCCTGTTTGATGATCCATCTCAGAGCGCCAATCTGCTGGCCGAAGCCAAAAAACTGAACGATGCCCAGGCCCCAAAA
Protein Sequence
MVDNKFNKEKTRAGAEIHSLPNLNVEQKFAFIVSLFDDPSQSANLLAEAKKLNDAQAPK
VC155 C-terminal fragment of mVenus for use i… 589..841 Rev
DNA Sequence
GACAAGCAGAAGAACGGCATCAAGGCAAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCAAACTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG
Protein Sequence
DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK