pTriEx-mCherry-LOV2 V416I

81045 Synthetic Bacterial Expression

Plasmid Information

Purpose
mammalian expression of LOV V416I
Depositor
Klaus Hahn
Article
Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n
Insert
LOV V416I
Tags
mCherry
Mutation
V416I
Available Since
Aug. 16, 2016
Availability
Available

Sequence Information

Length
986 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 2
Label Gene Product Position Strand
T7 promoter — — 363..382 Fwd
DNA Sequence
TAATACGACTCACTATAGG
p10 promoter — — 422..532 Fwd
DNA Sequence
GACCTTTAATTCAACCCAACACAATATATTATAGTTAAATAAGAATTATTATCAAATCATTTGTATATTAATTAAAATACTATACTGTAAATTACATTTTATTTACAATC
protein_bind 1
Label Gene Product Position Strand
lac operator — — 382..407 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC